Base: |
Plasmid collection |
Number in base: |
1674 |
Plasmid name: |
pRS307 |
Species: |
E. coli |
Strain: |
XL1 Blue MRF´ |
Genotype: |
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F´ proAB lacIqZΔM15 Tn10(TetR)]
|
Plasmid Sequence: |
>pRS307 8219 bp
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgca ccatatagct tcgcaagtat tcattttaga
361 cccatggtgg aaccctagtg ttgaatggca aagtggtgat agagttcata gaattggtca
421 gtatcgacct gtgaagatca caaggttttg cattgaggat agtatagaag caagaatcat
481 tgaattacag gaaaaaaagg caaatatgat tcatgctaca ataaaccaag atgaagctgc
541 cattagcaga ctaacgccag ctgatttaca gttcttattc aataactaat attttattct
601 cttattatat attattctcg gagtttttaa gtgacatcac ccgaaaagaa gctaagtctt
661 tctcctaatt catatttaat tattgtacat ggacatatca tacgtaatgc tcaaccttaa
721 gctgctgcgg agcttccacg agcaccagca cctgaagcaa ctagacttat ttgcgcttga
781 gttagttcta tacttggcag tggaagtttg tcattatgag ttggtggagg taaaaatcca
841 accttgttta aaaatgcaat atatatacca acctcttctg gagtaacacc tattccatta
901 gaccaatcaa cacctgtcca tgcggtgtct ttctttaaag atgccacggc gttcctatcg
961 tctagttccg gagctttggt actttcaggt aagttgtcta agaccatgtg tagcaaagga
1021 tacaacgcat tttcttcatt cctgtcaata acagacgcct ccaatgattt cttccattta
1081 gaatagcttt cgatttcgac atcgtaacca taatcgtgta aagtatacaa gtagtctttg
1141 aataatattc ttgggtgacc cgttacttga gcaacggcca attcattttc tttgggagga
1201 ttcaaagacg tagcaacaac aacacgagca acatgatcta ctggaaccat attcacggaa
1261 ttttcgatat ctggaatctt acctaattgg actgaacctt tcaaaaatct caataagaaa
1321 tcatctgtgt ttgaagaacc attggcagag gcacctgtta cgtaacctgg tctgacaata
1381 cacccacgta ggcccctttc acctgcacgt ctaatgatgt actcagcagc ccatttggac
1441 tgaccatatc caccagtgag cccgcttgca gagttcatta aatcgtctga ttctaaaatg
1501 cctggcttcc cttcgctaac aagtttatct gacaaattaa agtagtattc agtgtcaaga
1561 gtagaagtgg aggaaacaaa gtcaaagaac tttggcttgc cgacggcggc taagctcata
1621 acattgatag ttgaaataac atttggatcc ctcaatttgg catatggata aacccagtga
1681 actaacgcac cattatggat aattatatca actgtgtttg ccaaatccat ccacttctca
1741 tctgaaagac caaattggct tttagataaa tcgcctaata caactttaat atttgaggca
1801 aatttttcgt tccaagtacc ataggtgata cctgcctttt gtaatcttgc aaatgcagct
1861 tcttcatcct tggccctgac gtgggcaaac actttgaaac tgtagttctt tggagaacgt
1921 cctaacaaat ctgcaaggat gtaggagccc agaaatcctg tgacaccggt aacaaacaca
1981 ttaattgttg tttttccttc ggcactatta ggttcaacaa aatattctcg agaggggtac
2041 gaacttggta gcgtctcaac caatttcttg gcgtcttccg cataatttgc agtgacattt
2101 tcgacgacct caccttgaga tgatccaccc gatgatttaa ttctgtcaat ttccgcggca
2161 aaggccttta tcgttggata cttgaaaatt gtgcccaatg gtaaatcaac ttgcagcttt
2221 ttctttaagg taaaaatcat tttggtagcc aagatagaat gaccacctaa atcgaaaaac
2281 gaatcatctg gtgatacaga tgctggcttg gtaggtaata tacttaacca taagtctcta
2341 acctcgcgct caacattggt aaactgagag tcgtcagttt cagaaactgt attttcagct
2401 accaaattta attgcttggg agttgggaat tgaagtttag gcttatcaac tttaccattt
2461 ggattcaatg gtagtttatc cataaccaca atcaaggaag gcatagcata gctagccaat
2521 cttttcttta agaaagtcct gatgtccttg gataaaagat ggtaaccgat taagccctta
2581 actatagggt cagtttcaac ctcctttgga acatcacttt ggaacttaga caagtcatct
2641 ggcttgtcaa atcttgggac cataaatgtg atcaatgttg gctcattgtc ggcatttttg
2701 cgaactaaag taatgttttc tcttaccaat ggatgttggg aaatgtgcgt atctatttct
2761 cctaattcga ttctgaaccc acgaatttta acttgatcat cagccctacc gcaacattca
2821 cagtcaccgt ttggtagata acgacctaaa tcacccgttc tgtacaatct atctcttgga
2881 cctaaccaga attgtctcca aggttcacca ttatccttat ccaaataatt ccagtgatct
2941 ttttcaacaa accagttgtt cacaaatttt tctttattca attctggtaa tcctctataa
3001 ccttcggcca aaccacctgc acgaacataa atctcaccta tttcgccaat accacatatt
3061 tgagtacgat cgttcctgtt aacaactagt agctgaacgt tcaacatacc tttaccagca
3121 ggcatgacat ctttcaattt tttcaaaaag tttggatcgt cattttttga tttaacttcg
3181 aaataagaaa ctgcacgctg tgtttcagtg gtaccgtaca tattaacaat acggcaattt
3241 tctgccaagg tttgtaacct cagacaatca cgttttgtta aaatgtcacc cacaaagaac
3301 gcatgatgta acttagggaa tggtgtagta gcttgggcag taagtaattg acccatggca
3361 ggtgttaaat gggtaactgt gcaaccatac ttactcatcc attccgctaa acggcccggt
3421 gtaccaatat catcttgagt agggacatac aattgggcac ctaaaaataa tggtgtaaac
3481 atatctcttt gaattggatc atgtgcaata ccgctcagca ttgtgaattt atcattttct
3541 gttaagttga accttttgga catccaattg aaataataag ccaaggaaaa atgtctacca
3601 agaacaccct taggaatacc ttcggaacca gatgtgaaag atagggttgg gttggaatct
3661 ggtccaacta caacacctgt tctggtgtct ttgtagtgat catatggagc caaaacatcc
3721 tcgccattgt ccaatttgcc accttcaatg gtaccatttt cttgaatagc gatggaattg
3781 attcttgaaa caatctccaa ttcatcattg atgtaatctt ctactagttg atccaattgt
3841 ccagcagctc taataacaat caacccacgt ggtttagcaa cacctaagta aatggtttgt
3901 ctggctgggg gatatgcagg gtcgataact gaaaaggttg cgccggcttt caagacaccc
3961 atcacacata ccatcaaatc cacaccccta gaagaataga tcatcactac atcacctctt
4021 ttgatacctg ttttaatcaa ataatgggca actatgttag aagtgcggtt gatgtcgcga
4081 taagtgaaag aacgggactt gtcggaattt agtgttggag tctccacaac acaggttctc
4141 tctgggaagg cttcagcatt gtcctggaaa atgtcgtgaa tacaccccac gaaatcgcac
4201 cagcccaagt tcttagttgg atcaggtaaa ctatccttgg atgatgcggt gatcagagag
4261 attttagtta tgcaattgga tggatcgctt agcgcagcag tcaaatattg agtaaattgg
4321 tccgcaacaa tggttactct ttcattcgaa tacagtaagc tgttataaat taagttcaaa
4381 acatgcgcat tattactggt atccaaattc aaagcaaagt ccactaaatg atgcttgaac
4441 tcgtctaatt tgaaatcttg gttttccaaa aaggccaaac ggaacaactg aggggtcctt
4501 tccagatctt ggcaactttg aattttttca gctagctcgt caaaggaaaa attggcctca
4561 atagaattga gcttgttcaa ctcattgtta attgtagaat acagctcatt aaatgaccac
4621 gttggttgaa tattgaatct taagattttg ttattcgcaa tataaagaac aatatcatcg
4681 tcaccggtta ctctatatat caatgcagcc catacactca aagcgacagc gtatttgtta
4741 gaaaaactat catgaggcac atcgagctga ggtagctgta acgaatatgt agcttgtttc
4801 gtataaggtt cttgttgtgg gcgtaaaaag tcatgtggta acactgaaag agttggatta
4861 tccaacttct ctatccagac cttttcgtta gtcattagtg agtaactctg tgatatctct
4921 ctataattag cagtttttca ctgaaattcc aggaaaggta ataaactcag attttttttt
4981 atactattgg ctgcttgtta cttatatatc ttgaacttct cccagcgggt cttcaaatac
5041 atttgggcga tgttcatgtt cattaggcag gtatttcgac attgagtcac acgcgaaaaa
5101 ccgccggaat tttttatgta attgcaagtg gaattatatg cggtgtgaaa taccgcacag
5161 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
5221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
5281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
5341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
5401 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
5461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
5521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
5581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
5641 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
5701 tcttcgctat tacgccagct ggcgaagggg ggatgtgctg caaggcgatt aagttgggta
5761 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
5821 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc
5881 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
5941 ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
6001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg
6061 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt
6121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
6181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
6241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
6301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
6361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tcggcccccc
6421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
6481 aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
6541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
6601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
6661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
6721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
6781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
6841 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
6901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
6961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
7021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
7081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
7141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
7201 tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga
7261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
7321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
7381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
7441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
7501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
7561 catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
7621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
7681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
7741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
7801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
7861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
7921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
7981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
8041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
8101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
8161 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
|
Construction: |
The pRS307 is an yeast integrating (YIp) vector with a LYS2 selectable marker. It is a general purpose cloning plasmid for use in Saccharomyces cerevisiae, and basing on pRS backbone.
|
Plasmid properties/Comments: |
|
Plasmid map: |
|
Selectable Markers: |
AmpR LYS2 |
Antibiotic concetration: |
|
Plasmid size: |
8219 bp |
Origin of replication/Incompatibility group: |
|
Host range: |
E. coli |
Other genetic elements: |
|
Strain properties/Comments: |
see also pRS317 and pRS327 |
Author: |
Eriksson et al. |
Availability: |
non restricted |
Patent: |
|
Depositor: |
Krzysztof FLIS |
Institution/Department: |
|
Deposition date: |
2004-12-22 20:00+01:00 |
References: |
Eriksson,P., Thomas,L.R., Thorburn,A. and Stillman,D.J.(2004) pRS yeast vectors with a LYS2 marker; BioTechniques 36: 212-213. |