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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 1675
Plasmid name: pRS317
Species: E. coli
Strain: XL1 Blue MRF´
Genotype: Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F´ proAB lacIqZΔM15 Tn10(TetR)]
Plasmid Sequence: >pRS317 8601 bp 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatagc ttcgcaagta ttcattttag acccatggtg gaaccctagt gttgaatggc 241 aaagtggtga tagagttcat agaattggtc agtatcgacc tgtgaagatc acaaggtttt 301 gcattgagga tagtatagaa gcaagaatca ttgaattaca ggaaaaaaag gcaaatatga 361 ttcatgctac aataaaccaa gatgaagctg ccattagcag actaacgcca gctgatttac 421 agttcttatt caataactaa tattttattc tcttattata tattattctc ggagttttta 481 agtgacatca cccgaaaaga agctaagtct ttctcctaat tcatatttaa ttattgtaca 541 tggacatatc atacgtaatg ctcaacctta agctgctgcg gagcttccac gagcaccagc 601 acctgaagca actagactta tttgcgcttg agttagttct atacttggca gtggaagttt 661 gtcattatga gttggtggag gtaaaaatcc aaccttgttt aaaaatgcaa tatatatacc 721 aacctcttct ggagtaacac ctattccatt agaccaatca acacctgtcc atgcggtgtc 781 tttctttaaa gatgccacgg cgttcctatc gtctagttcc ggagctttgg tactttcagg 841 taagttgtct aagaccatgt gtagcaaagg atacaacgca ttttcttcat tcctgtcaat 901 aacagacgcc tccaatgatt tcttccattt agaatagctt tcgatttcga catcgtaacc 961 ataatcgtgt aaagtataca agtagtcttt gaataatatt cttgggtgac ccgttacttg 1021 agcaacggcc aattcatttt ctttgggagg attcaaagac gtagcaacaa caacacgagc 1081 aacatgatct actggaacca tattcacgga attttcgata tctggaatct tacctaattg 1141 gactgaacct ttcaaaaatc tcaataagaa atcatctgtg tttgaagaac cattggcaga 1201 ggcacctgtt acgtaacctg gtctgacaat acacccacgt aggccccttt cacctgcacg 1261 tctaatgatg tactcagcag cccatttgga ctgaccatat ccaccagtga gcccgcttgc 1321 agagttcatt aaatcgtctg attctaaaat gcctggcttc ccttcgctaa caagtttatc 1381 tgacaaatta aagtagtatt cagtgtcaag agtagaagtg gaggaaacaa agtcaaagaa 1441 ctttggcttg ccgacggcgg ctaagctcat aacattgata gttgaaataa catttggatc 1501 cctcaatttg gcatatggat aaacccagtg aactaacgca ccattatgga taattatatc 1561 aactgtgttt gccaaatcca tccacttctc atctgaaaga ccaaattggc ttttagataa 1621 atcgcctaat acaactttaa tatttgaggc aaatttttcg ttccaagtac cataggtgat 1681 acctgccttt tgtaatcttg caaatgcagc ttcttcatcc ttggccctga cgtgggcaaa 1741 cactttgaaa ctgtagttct ttggagaacg tcctaacaaa tctgcaagga tgtaggagcc 1801 cagaaatcct gtgacaccgg taacaaacac attaattgtt gtttttcctt cggcactatt 1861 aggttcaaca aaatattctc gagaggggta cgaacttggt agcgtctcaa ccaatttctt 1921 ggcgtcttcc gcataatttg cagtgacatt ttcgacgacc tcaccttgag atgatccacc 1981 cgatgattta attctgtcaa tttccgcggc aaaggccttt atcgttggat acttgaaaat 2041 tgtgcccaat ggtaaatcaa cttgcagctt tttctttaag gtaaaaatca ttttggtagc 2101 caagatagaa tgaccaccta aatcgaaaaa cgaatcatct ggtgatacag atgctggctt 2161 ggtaggtaat atacttaacc ataagtctct aacctcgcgc tcaacattgg taaactgaga 2221 gtcgtcagtt tcagaaactg tattttcagc taccaaattt aattgcttgg gagttgggaa 2281 ttgaagttta ggcttatcaa ctttaccatt tggattcaat ggtagtttat ccataaccac 2341 aatcaaggaa ggcatagcat agctagccaa tcttttcttt aagaaagtcc tgatgtcctt 2401 ggataaaaga tggtaaccga ttaagccctt aactataggg tcagtttcaa cctcctttgg 2461 aacatcactt tggaacttag acaagtcatc tggcttgtca aatcttggga ccataaatgt 2521 gatcaatgtt ggctcattgt cggcattttt gcgaactaaa gtaatgtttt ctcttaccaa 2581 tggatgttgg gaaatgtgcg tatctatttc tcctaattcg attctgaacc cacgaatttt 2641 aacttgatca tcagccctac cgcaacattc acagtcaccg tttggtagat aacgacctaa 2701 atcacccgtt ctgtacaatc tatctcttgg acctaaccag aattgtctcc aaggttcacc 2761 attatcctta tccaaataat tccagtgatc tttttcaaca aaccagttgt tcacaaattt 2821 ttctttattc aattctggta atcctctata accttcggcc aaaccacctg cacgaacata 2881 aatctcacct atttcgccaa taccacatat ttgagtacga tcgttcctgt taacaactag 2941 tagctgaacg ttcaacatac ctttaccagc aggcatgaca tctttcaatt ttttcaaaaa 3001 gtttggatcg tcattttttg atttaacttc gaaataagaa actgcacgct gtgtttcagt 3061 ggtaccgtac atattaacaa tacggcaatt ttctgccaag gtttgtaacc tcagacaatc 3121 acgttttgtt aaaatgtcac ccacaaagaa cgcatgatgt aacttaggga atggtgtagt 3181 agcttgggca gtaagtaatt gacccatggc aggtgttaaa tgggtaactg tgcaaccata 3241 cttactcatc cattccgcta aacggcccgg tgtaccaata tcatcttgag tagggacata 3301 caattgggca cctaaaaata atggtgtaaa catatctctt tgaattggat catgtgcaat 3361 accgctcagc attgtgaatt tatcattttc tgttaagttg aaccttttgg acatccaatt 3421 gaaataataa gccaaggaaa aatgtctacc aagaacaccc ttaggaatac cttcggaacc 3481 agatgtgaaa gatagggttg ggttggaatc tggtccaact acaacacctg ttctggtgtc 3541 tttgtagtga tcatatggag ccaaaacatc ctcgccattg tccaatttgc caccttcaat 3601 ggtaccattt tcttgaatag cgatggaatt gattcttgaa acaatctcca attcatcatt 3661 gatgtaatct tctactagtt gatccaattg tccagcagct ctaataacaa tcaacccacg 3721 tggtttagca acacctaagt aaatggtttg tctggctggg ggatatgcag ggtcgataac 3781 tgaaaaggtt gcgccggctt tcaagacacc catcacacat accatcaaat ccacacccct 3841 agaagaatag atcatcacta catcacctct tttgatacct gttttaatca aataatgggc 3901 aactatgtta gaagtgcggt tgatgtcgcg ataagtgaaa gaacgggact tgtcggaatt 3961 tagtgttgga gtctccacaa cacaggttct ctctgggaag gcttcagcat tgtcctggaa 4021 aatgtcgtga atacacccca cgaaatcgca ccagcccaag ttcttagttg gatcaggtaa 4081 actatccttg gatgatgcgg tgatcagaga gattttagtt atgcaattgg atggatcgct 4141 tagcgcagca gtcaaatatt gagtaaattg gtccgcaaca atggttactc tttcattcga 4201 atacagtaag ctgttataaa ttaagttcaa aacatgcgca ttattactgg tatccaaatt 4261 caaagcaaag tccactaaat gatgcttgaa ctcgtctaat ttgaaatctt ggttttccaa 4321 aaaggccaaa cggaacaact gaggggtcct ttccagatct tggcaacttt gaattttttc 4381 agctagctcg tcaaaggaaa aattggcctc aatagaattg agcttgttca actcattgtt 4441 aattgtagaa tacagctcat taaatgacca cgttggttga atattgaatc ttaagatttt 4501 gttattcgca atataaagaa caatatcatc gtcaccggtt actctatata tcaatgcagc 4561 ccatacactc aaagcgacag cgtatttgtt agaaaaacta tcatgaggca catcgagctg 4621 aggtagctgt aacgaatatg tagcttgttt cgtataaggt tcttgttgtg ggcgtaaaaa 4681 gtcatgtggt aacactgaaa gagttggatt atccaacttc tctatccaga ccttttcgtt 4741 agtcattagt gagtaactct gtgatatctc tctataatta gcagtttttc actgaaattc 4801 caggaaaggt aataaactca gatttttttt tatactattg gctgcttgtt acttatatat 4861 cttgaacttc tcccagcggg tcttcaaata catttgggcg atgttcatgt tcattaggca 4921 ggtatttcga cattgagtca cacgcgaaaa accgccggaa ttttttatgt aattgcaagt 4981 ggaattatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga 5041 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5101 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5161 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5221 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5281 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5341 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 5401 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 5461 acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg 5521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaggg 5581 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 5641 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg 5701 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct 5761 tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag 5821 ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5881 cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct 5941 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6001 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6061 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6241 tgctggcgtt tttccatagg ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa 6301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgttcccc cctggaagct 6361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6421 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 6481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6661 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7081 tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7141 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg gttagctcct 7441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7981 ttccccgaaa agtgccacct ggtcggtcct tttcatcacg tgctataaaa ataattataa 8041 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag 8101 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac 8161 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct 8221 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat 8281 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg 8341 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc 8401 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt 8461 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg 8521 tcgatccgtc gacctgctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 8581 gtatcacgag gccctttcgt c
Construction: The pRS317 is an yeast centromere (YCp) vector with a LYS2 selectable marker. It is a general purpose cloning plasmid for use in Saccharomyces cerevisiae, and basing on pRS backbone.
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: AmpR LYS2
Antibiotic concetration:
Plasmid size: 8601 bp
Origin of replication/Incompatibility group:
Host range: E. coli / yeast shuttle vector
Other genetic elements:
Strain properties/Comments: see also pRS307 and pRS327
Author: Eriksson et al.
Availability: non restricted
Patent:
Depositor: Krzysztof FLIS
Institution/Department:
Deposition date: 2004-12-22 20:00+01:00
References: Eriksson,P., Thomas,L.R., Thorburn,A. and Stillman,D.J.(2004) pRS yeast vectors with a LYS2 marker; BioTechniques 36: 212-213.