Base: |
Plasmid collection |
Number in base: |
1675 |
Plasmid name: |
pRS317 |
Species: |
E. coli |
Strain: |
XL1 Blue MRF´ |
Genotype: |
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F´ proAB lacIqZΔM15 Tn10(TetR)]
|
Plasmid Sequence: |
>pRS317 8601 bp
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatagc ttcgcaagta ttcattttag acccatggtg gaaccctagt gttgaatggc
241 aaagtggtga tagagttcat agaattggtc agtatcgacc tgtgaagatc acaaggtttt
301 gcattgagga tagtatagaa gcaagaatca ttgaattaca ggaaaaaaag gcaaatatga
361 ttcatgctac aataaaccaa gatgaagctg ccattagcag actaacgcca gctgatttac
421 agttcttatt caataactaa tattttattc tcttattata tattattctc ggagttttta
481 agtgacatca cccgaaaaga agctaagtct ttctcctaat tcatatttaa ttattgtaca
541 tggacatatc atacgtaatg ctcaacctta agctgctgcg gagcttccac gagcaccagc
601 acctgaagca actagactta tttgcgcttg agttagttct atacttggca gtggaagttt
661 gtcattatga gttggtggag gtaaaaatcc aaccttgttt aaaaatgcaa tatatatacc
721 aacctcttct ggagtaacac ctattccatt agaccaatca acacctgtcc atgcggtgtc
781 tttctttaaa gatgccacgg cgttcctatc gtctagttcc ggagctttgg tactttcagg
841 taagttgtct aagaccatgt gtagcaaagg atacaacgca ttttcttcat tcctgtcaat
901 aacagacgcc tccaatgatt tcttccattt agaatagctt tcgatttcga catcgtaacc
961 ataatcgtgt aaagtataca agtagtcttt gaataatatt cttgggtgac ccgttacttg
1021 agcaacggcc aattcatttt ctttgggagg attcaaagac gtagcaacaa caacacgagc
1081 aacatgatct actggaacca tattcacgga attttcgata tctggaatct tacctaattg
1141 gactgaacct ttcaaaaatc tcaataagaa atcatctgtg tttgaagaac cattggcaga
1201 ggcacctgtt acgtaacctg gtctgacaat acacccacgt aggccccttt cacctgcacg
1261 tctaatgatg tactcagcag cccatttgga ctgaccatat ccaccagtga gcccgcttgc
1321 agagttcatt aaatcgtctg attctaaaat gcctggcttc ccttcgctaa caagtttatc
1381 tgacaaatta aagtagtatt cagtgtcaag agtagaagtg gaggaaacaa agtcaaagaa
1441 ctttggcttg ccgacggcgg ctaagctcat aacattgata gttgaaataa catttggatc
1501 cctcaatttg gcatatggat aaacccagtg aactaacgca ccattatgga taattatatc
1561 aactgtgttt gccaaatcca tccacttctc atctgaaaga ccaaattggc ttttagataa
1621 atcgcctaat acaactttaa tatttgaggc aaatttttcg ttccaagtac cataggtgat
1681 acctgccttt tgtaatcttg caaatgcagc ttcttcatcc ttggccctga cgtgggcaaa
1741 cactttgaaa ctgtagttct ttggagaacg tcctaacaaa tctgcaagga tgtaggagcc
1801 cagaaatcct gtgacaccgg taacaaacac attaattgtt gtttttcctt cggcactatt
1861 aggttcaaca aaatattctc gagaggggta cgaacttggt agcgtctcaa ccaatttctt
1921 ggcgtcttcc gcataatttg cagtgacatt ttcgacgacc tcaccttgag atgatccacc
1981 cgatgattta attctgtcaa tttccgcggc aaaggccttt atcgttggat acttgaaaat
2041 tgtgcccaat ggtaaatcaa cttgcagctt tttctttaag gtaaaaatca ttttggtagc
2101 caagatagaa tgaccaccta aatcgaaaaa cgaatcatct ggtgatacag atgctggctt
2161 ggtaggtaat atacttaacc ataagtctct aacctcgcgc tcaacattgg taaactgaga
2221 gtcgtcagtt tcagaaactg tattttcagc taccaaattt aattgcttgg gagttgggaa
2281 ttgaagttta ggcttatcaa ctttaccatt tggattcaat ggtagtttat ccataaccac
2341 aatcaaggaa ggcatagcat agctagccaa tcttttcttt aagaaagtcc tgatgtcctt
2401 ggataaaaga tggtaaccga ttaagccctt aactataggg tcagtttcaa cctcctttgg
2461 aacatcactt tggaacttag acaagtcatc tggcttgtca aatcttggga ccataaatgt
2521 gatcaatgtt ggctcattgt cggcattttt gcgaactaaa gtaatgtttt ctcttaccaa
2581 tggatgttgg gaaatgtgcg tatctatttc tcctaattcg attctgaacc cacgaatttt
2641 aacttgatca tcagccctac cgcaacattc acagtcaccg tttggtagat aacgacctaa
2701 atcacccgtt ctgtacaatc tatctcttgg acctaaccag aattgtctcc aaggttcacc
2761 attatcctta tccaaataat tccagtgatc tttttcaaca aaccagttgt tcacaaattt
2821 ttctttattc aattctggta atcctctata accttcggcc aaaccacctg cacgaacata
2881 aatctcacct atttcgccaa taccacatat ttgagtacga tcgttcctgt taacaactag
2941 tagctgaacg ttcaacatac ctttaccagc aggcatgaca tctttcaatt ttttcaaaaa
3001 gtttggatcg tcattttttg atttaacttc gaaataagaa actgcacgct gtgtttcagt
3061 ggtaccgtac atattaacaa tacggcaatt ttctgccaag gtttgtaacc tcagacaatc
3121 acgttttgtt aaaatgtcac ccacaaagaa cgcatgatgt aacttaggga atggtgtagt
3181 agcttgggca gtaagtaatt gacccatggc aggtgttaaa tgggtaactg tgcaaccata
3241 cttactcatc cattccgcta aacggcccgg tgtaccaata tcatcttgag tagggacata
3301 caattgggca cctaaaaata atggtgtaaa catatctctt tgaattggat catgtgcaat
3361 accgctcagc attgtgaatt tatcattttc tgttaagttg aaccttttgg acatccaatt
3421 gaaataataa gccaaggaaa aatgtctacc aagaacaccc ttaggaatac cttcggaacc
3481 agatgtgaaa gatagggttg ggttggaatc tggtccaact acaacacctg ttctggtgtc
3541 tttgtagtga tcatatggag ccaaaacatc ctcgccattg tccaatttgc caccttcaat
3601 ggtaccattt tcttgaatag cgatggaatt gattcttgaa acaatctcca attcatcatt
3661 gatgtaatct tctactagtt gatccaattg tccagcagct ctaataacaa tcaacccacg
3721 tggtttagca acacctaagt aaatggtttg tctggctggg ggatatgcag ggtcgataac
3781 tgaaaaggtt gcgccggctt tcaagacacc catcacacat accatcaaat ccacacccct
3841 agaagaatag atcatcacta catcacctct tttgatacct gttttaatca aataatgggc
3901 aactatgtta gaagtgcggt tgatgtcgcg ataagtgaaa gaacgggact tgtcggaatt
3961 tagtgttgga gtctccacaa cacaggttct ctctgggaag gcttcagcat tgtcctggaa
4021 aatgtcgtga atacacccca cgaaatcgca ccagcccaag ttcttagttg gatcaggtaa
4081 actatccttg gatgatgcgg tgatcagaga gattttagtt atgcaattgg atggatcgct
4141 tagcgcagca gtcaaatatt gagtaaattg gtccgcaaca atggttactc tttcattcga
4201 atacagtaag ctgttataaa ttaagttcaa aacatgcgca ttattactgg tatccaaatt
4261 caaagcaaag tccactaaat gatgcttgaa ctcgtctaat ttgaaatctt ggttttccaa
4321 aaaggccaaa cggaacaact gaggggtcct ttccagatct tggcaacttt gaattttttc
4381 agctagctcg tcaaaggaaa aattggcctc aatagaattg agcttgttca actcattgtt
4441 aattgtagaa tacagctcat taaatgacca cgttggttga atattgaatc ttaagatttt
4501 gttattcgca atataaagaa caatatcatc gtcaccggtt actctatata tcaatgcagc
4561 ccatacactc aaagcgacag cgtatttgtt agaaaaacta tcatgaggca catcgagctg
4621 aggtagctgt aacgaatatg tagcttgttt cgtataaggt tcttgttgtg ggcgtaaaaa
4681 gtcatgtggt aacactgaaa gagttggatt atccaacttc tctatccaga ccttttcgtt
4741 agtcattagt gagtaactct gtgatatctc tctataatta gcagtttttc actgaaattc
4801 caggaaaggt aataaactca gatttttttt tatactattg gctgcttgtt acttatatat
4861 cttgaacttc tcccagcggg tcttcaaata catttgggcg atgttcatgt tcattaggca
4921 ggtatttcga cattgagtca cacgcgaaaa accgccggaa ttttttatgt aattgcaagt
4981 ggaattatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
5041 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
5101 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
5161 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
5221 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
5281 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
5341 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
5401 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
5461 acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg
5521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaggg
5581 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
5641 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg
5701 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
5761 tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag
5821 ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
5881 cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct
5941 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
6001 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
6061 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
6121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
6181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
6241 tgctggcgtt tttccatagg ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa
6301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgttcccc cctggaagct
6361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
6421 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
6481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
6541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
6601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
6661 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
6721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
6781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
6841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
6901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
6961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
7021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
7081 tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
7141 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
7201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
7261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
7321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
7381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg gttagctcct
7441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
7501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
7561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
7621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
7681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
7741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
7801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
7861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
7921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
7981 ttccccgaaa agtgccacct ggtcggtcct tttcatcacg tgctataaaa ataattataa
8041 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
8101 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
8161 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
8221 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
8281 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
8341 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
8401 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
8461 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
8521 tcgatccgtc gacctgctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
8581 gtatcacgag gccctttcgt c
|
Construction: |
The pRS317 is an yeast centromere (YCp) vector with a LYS2 selectable marker. It is a general purpose cloning plasmid for use in Saccharomyces cerevisiae, and basing on pRS backbone.
|
Plasmid properties/Comments: |
|
Plasmid map: |
|
Selectable Markers: |
AmpR LYS2 |
Antibiotic concetration: |
|
Plasmid size: |
8601 bp |
Origin of replication/Incompatibility group: |
|
Host range: |
E. coli / yeast shuttle vector |
Other genetic elements: |
|
Strain properties/Comments: |
see also pRS307 and pRS327 |
Author: |
Eriksson et al. |
Availability: |
non restricted |
Patent: |
|
Depositor: |
Krzysztof FLIS |
Institution/Department: |
|
Deposition date: |
2004-12-22 20:00+01:00 |
References: |
Eriksson,P., Thomas,L.R., Thorburn,A. and Stillman,D.J.(2004) pRS yeast vectors with a LYS2 marker; BioTechniques 36: 212-213. |