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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 1676
Plasmid name: pRS327
Species: E. coli
Strain: XL1 Blue MRF´
Genotype: Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F´ proAB lacIqZΔM15 Tn10(TetR)]
Plasmid Sequence: >pRS327 9560 bp 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgca ccatatagct tcgcaagtat tcattttaga 361 cccatggtgg aaccctagtg ttgaatggca aagtggtgat agagttcata gaattggtca 421 gtatcgacct gtgaagatca caaggttttg cattgaggat agtatagaag caagaatcat 481 tgaattacag gaaaaaaagg caaatatgat tcatgctaca ataaaccaag atgaagctgc 541 cattagcaga ctaacgccag ctgatttaca gttcttattc aataactaat attttattct 601 cttattatat attattctcg gagtttttaa gtgacatcac ccgaaaagaa gctaagtctt 661 tctcctaatt catatttaat tattgtacat ggacatatca tacgtaatgc tcaaccttaa 721 gctgctgcgg agcttccacg agcaccagca cctgaagcaa ctagacttat ttgcgcttga 781 gttagttcta tacttggcag tggaagtttg tcattatgag ttggtggagg taaaaatcca 841 accttgttta aaaatgcaat atatatacca acctcttctg gagtaacacc tattccatta 901 gaccaatcaa cacctgtcca tgcggtgtct ttctttaaag atgccacggc gttcctatcg 961 tctagttccg gagctttggt actttcaggt aagttgtcta agaccatgtg tagcaaagga 1021 tacaacgcat tttcttcatt cctgtcaata acagacgcct ccaatgattt cttccattta 1081 gaatagcttt cgatttcgac atcgtaacca taatcgtgta aagtatacaa gtagtctttg 1141 aataatattc ttgggtgacc cgttacttga gcaacggcca attcattttc tttgggagga 1201 ttcaaagacg tagcaacaac aacacgagca acatgatcta ctggaaccat attcacggaa 1261 ttttcgatat ctggaatctt acctaattgg actgaacctt tcaaaaatct caataagaaa 1321 tcatctgtgt ttgaagaacc attggcagag gcacctgtta cgtaacctgg tctgacaata 1381 cacccacgta ggcccctttc acctgcacgt ctaatgatgt actcagcagc ccatttggac 1441 tgaccatatc caccagtgag cccgcttgca gagttcatta aatcgtctga ttctaaaatg 1501 cctggcttcc cttcgctaac aagtttatct gacaaattaa agtagtattc agtgtcaaga 1561 gtagaagtgg aggaaacaaa gtcaaagaac tttggcttgc cgacggcggc taagctcata 1621 acattgatag ttgaaataac atttggatcc ctcaatttgg catatggata aacccagtga 1681 actaacgcac cattatggat aattatatca actgtgtttg ccaaatccat ccacttctca 1741 tctgaaagac caaattggct tttagataaa tcgcctaata caactttaat atttgaggca 1801 aatttttcgt tccaagtacc ataggtgata cctgcctttt gtaatcttgc aaatgcagct 1861 tcttcatcct tggccctgac gtgggcaaac actttgaaac tgtagttctt tggagaacgt 1921 cctaacaaat ctgcaaggat gtaggagccc agaaatcctg tgacaccggt aacaaacaca 1981 ttaattgttg tttttccttc ggcactatta ggttcaacaa aatattctcg agaggggtac 2041 gaacttggta gcgtctcaac caatttcttg gcgtcttccg cataatttgc agtgacattt 2101 tcgacgacct caccttgaga tgatccaccc gatgatttaa ttctgtcaat ttccgcggca 2161 aaggccttta tcgttggata cttgaaaatt gtgcccaatg gtaaatcaac ttgcagcttt 2221 ttctttaagg taaaaatcat tttggtagcc aagatagaat gaccacctaa atcgaaaaac 2281 gaatcatctg gtgatacaga tgctggcttg gtaggtaata tacttaacca taagtctcta 2341 acctcgcgct caacattggt aaactgagag tcgtcagttt cagaaactgt attttcagct 2401 accaaattta attgcttggg agttgggaat tgaagtttag gcttatcaac tttaccattt 2461 ggattcaatg gtagtttatc cataaccaca atcaaggaag gcatagcata gctagccaat 2521 cttttcttta agaaagtcct gatgtccttg gataaaagat ggtaaccgat taagccctta 2581 actatagggt cagtttcaac ctcctttgga acatcacttt ggaacttaga caagtcatct 2641 ggcttgtcaa atcttgggac cataaatgtg atcaatgttg gctcattgtc ggcatttttg 2701 cgaactaaag taatgttttc tcttaccaat ggatgttggg aaatgtgcgt atctatttct 2761 cctaattcga ttctgaaccc acgaatttta acttgatcat cagccctacc gcaacattca 2821 cagtcaccgt ttggtagata acgacctaaa tcacccgttc tgtacaatct atctcttgga 2881 cctaaccaga attgtctcca aggttcacca ttatccttat ccaaataatt ccagtgatct 2941 ttttcaacaa accagttgtt cacaaatttt tctttattca attctggtaa tcctctataa 3001 ccttcggcca aaccacctgc acgaacataa atctcaccta tttcgccaat accacatatt 3061 tgagtacgat cgttcctgtt aacaactagt agctgaacgt tcaacatacc tttaccagca 3121 ggcatgacat ctttcaattt tttcaaaaag tttggatcgt cattttttga tttaacttcg 3181 aaataagaaa ctgcacgctg tgtttcagtg gtaccgtaca tattaacaat acggcaattt 3241 tctgccaagg tttgtaacct cagacaatca cgttttgtta aaatgtcacc cacaaagaac 3301 gcatgatgta acttagggaa tggtgtagta gcttgggcag taagtaattg acccatggca 3361 ggtgttaaat gggtaactgt gcaaccatac ttactcatcc attccgctaa acggcccggt 3421 gtaccaatat catcttgagt agggacatac aattgggcac ctaaaaataa tggtgtaaac 3481 atatctcttt gaattggatc atgtgcaata ccgctcagca ttgtgaattt atcattttct 3541 gttaagttga accttttgga catccaattg aaataataag ccaaggaaaa atgtctacca 3601 agaacaccct taggaatacc ttcggaacca gatgtgaaag atagggttgg gttggaatct 3661 ggtccaacta caacacctgt tctggtgtct ttgtagtgat catatggagc caaaacatcc 3721 tcgccattgt ccaatttgcc accttcaatg gtaccatttt cttgaatagc gatggaattg 3781 attcttgaaa caatctccaa ttcatcattg atgtaatctt ctactagttg atccaattgt 3841 ccagcagctc taataacaat caacccacgt ggtttagcaa cacctaagta aatggtttgt 3901 ctggctgggg gatatgcagg gtcgataact gaaaaggttg cgccggcttt caagacaccc 3961 atcacacata ccatcaaatc cacaccccta gaagaataga tcatcactac atcacctctt 4021 ttgatacctg ttttaatcaa ataatgggca actatgttag aagtgcggtt gatgtcgcga 4081 taagtgaaag aacgggactt gtcggaattt agtgttggag tctccacaac acaggttctc 4141 tctgggaagg cttcagcatt gtcctggaaa atgtcgtgaa tacaccccac gaaatcgcac 4201 cagcccaagt tcttagttgg atcaggtaaa ctatccttgg atgatgcggt gatcagagag 4261 attttagtta tgcaattgga tggatcgctt agcgcagcag tcaaatattg agtaaattgg 4321 tccgcaacaa tggttactct ttcattcgaa tacagtaagc tgttataaat taagttcaaa 4381 acatgcgcat tattactggt atccaaattc aaagcaaagt ccactaaatg atgcttgaac 4441 tcgtctaatt tgaaatcttg gttttccaaa aaggccaaac ggaacaactg aggggtcctt 4501 tccagatctt ggcaactttg aattttttca gctagctcgt caaaggaaaa attggcctca 4561 atagaattga gcttgttcaa ctcattgtta attgtagaat acagctcatt aaatgaccac 4621 gttggttgaa tattgaatct taagattttg ttattcgcaa tataaagaac aatatcatcg 4681 tcaccggtta ctctatatat caatgcagcc catacactca aagcgacagc gtatttgtta 4741 gaaaaactat catgaggcac atcgagctga ggtagctgta acgaatatgt agcttgtttc 4801 gtataaggtt cttgttgtgg gcgtaaaaag tcatgtggta acactgaaag agttggatta 4861 tccaacttct ctatccagac cttttcgtta gtcattagtg agtaactctg tgatatctct 4921 ctataattag cagtttttca ctgaaattcc aggaaaggta ataaactcag attttttttt 4981 atactattgg ctgcttgtta cttatatatc ttgaacttct cccagcgggt cttcaaatac 5041 atttgggcga tgttcatgtt cattaggcag gtatttcgac attgagtcac acgcgaaaaa 5101 ccgccggaat tttttatgta attgcaagtg gaattatatg cggtgtgaaa taccgcacag 5161 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc 5221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 5281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 5341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 5401 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 5461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 5521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 5581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 5641 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 5701 tcttcgctat tacgccagct ggcgaagggg ggatgtgctg caaggcgatt aagttgggta 5761 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac 5821 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc 5881 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc 5941 ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc 6001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg 6061 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt 6121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tcggcccccc 6421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6481 aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 6601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6841 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7201 tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga 7261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7561 catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 8101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat 8161 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga 8221 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa 8281 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac 8341 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca 8401 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc 8461 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg 8521 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt 8581 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc 8641 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg 8701 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga 8761 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt 8821 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact 8881 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt 8941 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag 9001 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt 9061 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc 9121 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga 9181 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta 9241 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg 9301 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct 9361 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct 9421 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct 9481 ttgatattgg atcatctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9541 tatcacgagg ccctttcgtc
Construction: The pRS327 is an yeast episomal (YEp) vector with a LYS2 selectable marker. It is a general purpose cloning plasmid for use in Saccharomyces cerevisiae, and basing on pRS backbone.
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: AmpR LYS2
Antibiotic concetration:
Plasmid size: 9560 bp
Origin of replication/Incompatibility group:
Host range: E. coli / yeast shuttle vector
Other genetic elements:
Strain properties/Comments: see also pRS307 and pRS317
Author: Eriksson et al.
Availability: non restricted
Patent:
Depositor: Krzysztof FLIS
Institution/Department:
Deposition date: 2004-12-22 20:00+01:00
References: Eriksson,P., Thomas,L.R., Thorburn,A. and Stillman,D.J.(2004) pRS yeast vectors with a LYS2 marker; BioTechniques 36: 212-213.