| Base: |
Plasmid collection |
| Number in base: |
1744 |
| Plasmid name: |
pET28Δ31korB |
| Species: |
E. coli |
| Strain: |
BL21(DE3) |
| Genotype: |
F- ompT hsdSB(rB-mB-) gal dcm (λDE3) |
| Plasmid Sequence: |
no available |
| Construction: |
pET28 derivative with EcoRI-SalI fragment of korBRK2 encoding internal 33aa of the protein (NΔ255-CΔ78aa) inserted downstream of a His6-tag |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Kan |
| Antibiotic concetration: |
|
| Plasmid size: |
5,6 kb |
| Origin of replication/Incompatibility group: |
pMBI |
| Host range: |
narrow |
| Other genetic elements: |
His-tag, trombin cleavege site |
| Strain properties/Comments: |
T7 polymerase |
| Author: |
Lukaszewicz M. |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Bartosik Aneta |
| Institution/Department: |
|
| Deposition date: |
2006-06-26 14:20+01:00 |
| References: |
Lukaszewicz, M. et al., 2000 |