| Base: |
Plasmid collection |
| Number in base: |
2128 |
| Plasmid name: |
AGG1,AGB1/pBridge |
| Species: |
E. coli |
| Strain: |
XL-1 Blue MRF’ |
| Genotype: |
∆(mcrA)183 ∆(mcrBC-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1lac [F’ proAB lacIq Z∆M15 Tn10 (Tetr)] |
| Plasmid Sequence: |
a |
| Construction: |
The 306 bp cDNA of AGG1 gene from Arabidopsis thaliana was cloned into pBridge vector using NotI and BglII restriction sites into MCS2. The The first methionine ATG codon was removed. The 1137 bp cDNA of AGB1 gene from Arabidopsis thaliana was cloned into pBridge vector using EcoRI and EcoRI restriction sites into MCS1. The The first methionine ATG codon was removed. |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Amp, TRP1 |
| Antibiotic concetration: |
|
| Plasmid size: |
|
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
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| Strain properties/Comments: |
|
| Author: |
Kamil Olejnik |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Kamil Olejnik |
| Institution/Department: |
|
| Deposition date: |
2008-04-11 12:29+01:00 |
| References: |
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