| Base: |
Plasmid collection |
| Number in base: |
247 |
| Plasmid name: |
pYEF1PVYhel |
| Species: |
Escherichia coli |
| Strain: |
TOP10F` |
| Genotype: |
F`{ lacIq Tn10(TetR )} mcrA D( mrr-hsdRMS- mcrBC) F80 lacZDM15 D lacX74 deoR recA1 araD139 D( ara-leu)7697 galU galK rpsL endA1 nupG |
| Plasmid Sequence: |
|
| Construction: |
derivative of pYEF1, 1900 bp insert encoding RNA helicase of PVY virus with C-terminal 6xHis epitope; cloned by using homologic recombination method; pYEFJA2His digested with BstEII and SacII enzymes |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
8974 bp |
| Origin of replication/Incompatibility group: |
2 &mikro;m |
| Host range: |
E.coli, S. cerevisiae |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Boguszewska-Chachulska A.M.,Gozdek A. |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Iwona Rosa |
| Institution/Department: |
|
| Deposition date: |
29.03.2002 |
| References: |
Trends Genet. 2001 Jun. 17(6):306-308 Dessen P. i.in.; http://paramecium.cgm.cnrs-gif.fr |