α β γ δ ε ζ η θ ι κ λ μ ν ξ ο π ρ Σ σ τ υ φ χ ψ ω Δ Γ Ω Σ Λ Ψ Φ Ξ Θ Π °C Greek letters

Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 2530
Plasmid name: pGJ2
Species: Escherichia coli
Strain: DH5α
Genotype: φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169
Plasmid Sequence: TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC ACCATAATTCCGTTTTAAGAGCTTGGTGAGCGCTAGGAGTCACTGCCAGGTATCGTTTGA ACACGGCATTAGTCAGGGAAGTCATAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATT ACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTT TTTTTTTTCCACCTAGCGGATGACTCTTTTTTTTTCTTAGCGATTGGCATTATCACATAA TGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCA GGCAAGATAAACGAAGGCAAAGATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAA TGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTC GATCTTCCCAGAAAAAGAGGCAGAAGCAGTAGCAGAACAGGCCACACAATCGCAAGTGAT TAACGTCCACACAGGTATAGGGTTTCTGGACCATATGATACATGCTCTGGCCAAGCATTC CGGCTGGTCGCTAATCGTTGAGTGCATTGGTGACTTACACATAGACGACCATCACACCAC TGAAGACTGCGGGATTGCTCTCGGTCAAGCTTTTAAAGAGGCCCTACTGGCGCGTGGAGT AAAAAGGTTTGGATCAGGATTTGCGCCTTTGGATGAGGCACTTTCCAGAGCGGTGGTAGA TCTTTCGAACAGGCCGTACGCAGTTGTCGAACTTGGTTTGCAAAGGGAGAAAGTAGGAGA TCTCTCTTGCGAGATGATCCCGCATTTTCTTGAAAGCTTTGCAGAGGCTAGCAGAATTAC CCTCCACGTTGATTGTCTGCGAGGCAAGAATGATCATCACCGTAGTGAGAGTGCGTTCAA GGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTC CACCAAAGGTGTTCTTATGTAGTGACACCGATTATTTAAAGCTGCAGCATACGATATATA TACATGTGTATATATGTATACCTATGAATGTCAGTAAGTATGTATACGAACAGTATGATA CTGAAGATGACAAGGTAATGCATCATTCTATACGTGTCATTCTGAACGAGGCGCGCTTTC CTTTTTTCTTTTTGCTTTTTCTTTTTTTTTCTCTTGAACTCGACGGATCATATGCGGTGT GAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATA TTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCG AAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTC CAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGT CGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGAC GGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTA GGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATG CGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGC GATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAGGGGGGATGTGCTGCAAGGC GATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTG AATTGTAATACGACTCACTATAGGGCGAATTGGAGCTCCACCGCGGTGGCGGCCGCTCTA GAACTAGTGGATCCCATATATTGTATGCCGAAATCCGTTACATCAAAATTCAAAAAGTAT CCATACAAATAGATGGCAGAGTACCAACACTACCGTGCTTGTTTAAGTGTTCTTATTTTG ATGCATTATTTAGATATATATCTCTTCTTGTATAGTTTTCTTTGAGGATGGAACGTACTC CATATACCTAGCACGTCTATTATGAATGAAACTTCCAGGGTTAATTACTTTACAACCGTT GTATGTCAAATCAAATTGTGCAGAAGTAGTGTCGCAAAGAACCATTGTCGATGGTATTGG GCAAAGTGTCAAAGTGTGGTCCAAATCCCATGAAATTGGGCGCAAGGAATCAAGAAATGG CGATAAATGACCCTGGTCTAGTATTGTTTTAACGAGTTTTCTTGTTTCTTGAACCTTTTG TGGTAACTGGTCTGGTTCTTTAACTAATTCATCGATTGGTACAATATCGGTGTCTTTAGA CATCATGTTATCGTTTTCAGTATAAACATCTTCGCTCTCGTTGAATGGGAATTCCAAACG GTGTCTTTTGAATCTTCCGGATAAATCGTCCCTGAAAATGACTATTTCTTGGGATAAGTA TGCTATTCTAGTTGGATTTGAGCTCCATACAACGTTTTTACAGACCTTGTTGATTTTTTT GGTAAACGCACTAGGAATTGGATCTTGCGGTAATGTCCCACTTGCCCCCAATGACACCAT CGACCCCCATAAATCGTTAGGACCTGGAATAAATATCATTGTGGTATTTTCGGTCAAATT GTCAAATCTTGACAGAAGCGTCGCCAAGGCATCGAAATTATTCTTAAATTGAGTGGAACT ACTTATATTCCGGCTACTCATTGATGCGAAAACGGGAACTGAAGTGAAAGAACCTTGCCA AATTAATAGGGTCGGTGGGTCATCATTTAATTTTTGCAAAATTTTGCTGAGTGCAGTCAT AATTTTCAAATCATCTAGGAACAAATTTGCGCCGAGAATTACAAATTTATGATCTGTTAA TTCTTTCTCCAAAAGGTGTAATCTAATCTTCAAATCTTTATCCAAACGAGCAATGAAATT GTTATTAGAAATGCCATGTATTCCTAAGAGATCGAGATTACCTATCGTTTCTAATGTAAT TTCTCTTCTCTCACCAGGGGGTAAAGTCATGGAGGTAACATGGAATTTATTTCCTACAGA ATAGTATATTCCTTCCACAAGAACCATGCAACCTGGCACGTAGTAATGGCCTTGTGTAGG GATAGTTTGGGAAATGTCAATTTCGACAGATCCAGACGGATCTTCCAGTGACCAATTGCC CTTAAAGTTCTTATTTAAGAGCCCCAATAGTAGAAAGTTTTGAGCATCCCTACCTAGTAG GTTTTTTATTGGGGTGATACTCATACTGCTGGATTGCTGCTGTCGGTTAGTATTGGACAG TTCATTTTGTAAAGACACCATAGATGATAATGGATTAAACATGTCACTGTTTTGGAAGTT TTCATTTCTCATGACTCTGTCGTTCGTGAGGTAATATCTTGTCAAGAACATTTGAACTTT GTCCTCTATATCTGGTAGAAAACCCGCAATGCCTCCTAGCCCATTCTGCTTTTTATTGGG GACGAAAATAAATTGCATTTTGTGCGGATTGTATGAGAATCTTTGTTGCTGAGAAGCATT GATAACTTTGAAGTAGTCCCTCCAGTCCAACGCCTTGGAGCTTTCTGGCTTGAAAGGCTG TTTATATTCATCTCTTTCGGTGGGCTGTCGCATGGGTGAAGATAATGAAACATTTTGTAA GGATGAATCAGCAGCGATCGGCATTTCATCGTCACTGTTGTTCTCATCGTCATCTGTCCG TCCCAAGATATTCTCCTCATGTTGAATAGGGTGTTCATGACTCCATTCGACTTTCTCACG TTCTTTCATCTCCTGTATAACTTCTTTAACCCCGCTCTGGTCGATGAAAAGGCCTCTTTC TTGTTGTTTCCATACTGCAGCAAATTGCTCGAGAAATTTTATTGTAGCAGGCCCTTGTCT CCAGTTAGCACCTATATTAGTGCCAACGAATTCTGCAAGAGCAGACAGTCCATCAGATTT AATTGATAAACCATATTTTCTTGATAAAACTCTATACGCCAGTGGCCTAAGCAGCGGAGG CTGAATTTTAACAGGCAGAACATTCCCAGAGCCAAACATTTCACACATAGACGCTTCTAG TTCCACTTACAAACTTTAAAGGCATAAACAAGTCATCTCAACTTTATTGTAACATGTGTT AAAGATGCCCAGTTGAAATATTTTTCTATTATGTCACGCGAAAAATAACGCGTCACATCA CTTTTGTTCCTTTTGTCGTTTTTCTTCTTTTAGATTCCATCTTGAAAAGAGATGAAATGG ACAAAGTAACATAATTTAGACCCAACAAAGTAAAAAGAAGGTTGGGTTGGCATTGAAGAT CACAATGGGAATTCAGGAAAAGACATTGGGGATACGAAAAGAGCGCAAATTGGTGGTTGT GCCTCGCGAAAGAAATCATGTTAGACACGCTTCACAAAGGACACGTTCTAAAAACTACAA GAATATCTCCAAAAAGAGGGCTCAACAACACGCGTTTGGATTCAATATTGCTAAGACTCT TGCCAAGATCCAAGCGTTTGTATGGGGGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTT TTGTTCCCTTTAGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCC TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAGGAGCCGGAAGCATAAAGTG TAAAGCCTGGGGTGCCTAATGAGTGAGGTAACTCACATTAATTGCGTTGCGCTCACTGCC CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCGGCCCCCCTGACGAGCATCA CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC GTTCCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTA TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT CCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTC TGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTC ATCCATAGTTGCCTGACTGCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATC TGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGC AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGC TTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGAAA AAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTT ATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATG CTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACC GAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTT GAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTT CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAG GGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTA TCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAT AGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGTCCTTTTCATCACGTGCTAT AAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAA AAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGA TCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATT GTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTT ATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTA AAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTA ACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAA ACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTAC AGGCAAGCGATCCGTCCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGC GTATCACGAGGCCCTTTCGTC
Construction: the DPB2 gene was cloned with its natural promotor and terminator sequence to pRS313; DPB2 containing fragment was amplified using pMJDPB2 plasmid as a template and primers:5'-CATC TGCGGTCGACCCCCATACAAAC-3' (SalI underlined) and 5'-CAAAAAGTATGGATCCAAATAGATGGCAG-3' (BamHI underlined).
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: Amp
Antibiotic concetration:
Plasmid size: 7581 bp
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Malgorzata Jaszczur
Availability: restricted
Patent:
Depositor: Malgorzata Jaszczur
Institution/Department:
Deposition date: 2009-03-21 17:53+01:00
References: Jaszczur et al. (2008) Genetics 178:633-647