Base: |
Plasmid collection |
Number in base: |
2541 |
Plasmid name: |
pKF117dpb2-GJ6 |
Species: |
Escherichia coli |
Strain: |
DH5α |
Genotype: |
φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169 pKF117dpb2-GJ6
|
Plasmid Sequence: |
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
ACCATAATTCCGTTTTAAGAGCTTGGTGAGCGCTAGGAGTCACTGCCAGGTATCGTTTGA
ACACGGCATTAGTCAGGGAAGTCATAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATT
ACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTT
TTTTTTTTCCACCTAGCGGATGACTCTTTTTTTTTCTTAGCGATTGGCATTATCACATAA
TGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCA
GGCAAGATAAACGAAGGCAAAGATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAA
TGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTC
GATCTTCCCAGAAAAAGAGGCAGAAGCAGTAGCAGAACAGGCCACACAATCGCAAGTGAT
TAACGTCCACACAGGTATAGGGTTTCTGGACCATATGATACATGCTCTGGCCAAGCATTC
CGGCTGGTCGCTAATCGTTGAGTGCATTGGTGACTTACACATAGACGACCATCACACCAC
TGAAGACTGCGGGATTGCTCTCGGTCAAGCTTTTAAAGAGGCCCTACTGGCGCGTGGAGT
AAAAAGGTTTGGATCAGGATTTGCGCCTTTGGATGAGGCACTTTCCAGAGCGGTGGTAGA
TCTTTCGAACAGGCCGTACGCAGTTGTCGAACTTGGTTTGCAAAGGGAGAAAGTAGGAGA
TCTCTCTTGCGAGATGATCCCGCATTTTCTTGAAAGCTTTGCAGAGGCTAGCAGAATTAC
CCTCCACGTTGATTGTCTGCGAGGCAAGAATGATCATCACCGTAGTGAGAGTGCGTTCAA
GGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTC
CACCAAAGGTGTTCTTATGTAGTGACACCGATTATTTAAAGCTGCAGCATACGATATATA
TACATGTGTATATATGTATACCTATGAATGTCAGTAAGTATGTATACGAACAGTATGATA
CTGAAGATGACAAGGTAATGCATCATTCTATACGTGTCATTCTGAACGAGGCGCGCTTTC
CTTTTTTCTTTTTGCTTTTTCTTTTTTTTTCTCTTGAACTCGACGGATCATATGCGGTGT
GAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATA
TTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCG
AAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTC
CAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA
CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGT
CGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGAC
GGGGAAAGCCCTCGTTTTCGACACTGGATGGCGGCGTTAGTATCGAATCGACAGCAGTAT
AGCGACCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGCACTTAACTTCGC
ATCTGGGCAGATGATGTCGAGGCGAAAAAAAATATAAATCACGCTAACATTTGATTAAAA
TAGAACAACTACAATATAAAAAAACTATACAAATGACAAGTTCTTGAAAACAAGAATCTT
TTTATTGTCAGTACTGATTATAATTGGCCAGTCTTTTTCAAATAAGCATTCCAACCAGCA
TCTCTATACCTTTTACCTTCAATATCTGGATCTCTTCCTTTACCAAACAATCCTCTACCA
ACAATGATAATATCAGTTCCAGTGCTAACAACTTCATCAACAGTTCTATATTGTTGTCCT
AATCCATCACCTTTATCATCTAATCCAACTCCAGGTGTCATAATAAGCCAATCAAATCCT
TCTTCTTGTCCACCCATATCACGTTGGGCAATAAATCCAATAACAAATTCCTTATCGGAT
TTAGCAATTTCAACAGTTTTTTGAGAATATTCTCCATATGCTAATGATCCCACTGATGAT
AATTCAGCTAACATCAATAACCCTCTTGGCTCTTGGTTGGTGGTGGTTTCTTTAGCTCCC
TGTTTTAATCCTTCAACTACTCCATTCCCAGTGACACCATGAGCATTAGTAATATCTGCC
CAACTACTAATTTTATAAACTCCACCAATATATTGTTTCTTCACGGTATTACCAATATCA
GCAAATTTTCTATCTTCAAAAATCATAAATTGATGTTTACGTGAAAGTTCTAATAATGGT
TCAATAGTGGATTCATAGGAAAAATCATTGATTATATCAATATGAGTCTTGATTAAGCAT
ACATAAGGACCCAATTTATCAATTAATTCAAGGAATTCCTTAGTGGTATCAACATCAATT
GATGCACATAAATTGGTTTTCTTCAGTTCCATTAATCGAAATAATCGTTGTGCTACTGGT
GAGGCATGAGTTTCTGCTCTCTCACTATAGGTCTTAGTGTTGACTGTCATGGTTGTTTAT
GTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGA
AGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGAC
AATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGT
AATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGG
GATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAA
GACTGTCAAGGAGGGTATTCTGGGCCTCCATGTCGCTGGCCGGGTGACCCGGCGGGGACA
AGGCAAGCTAAACAGATCTGGCGCGCCTTAATTAACCCGGCGAACGTGGCGAGAAAGGAA
GGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGC
GTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCA
TTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG
CTGGCGAAGGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGTTTTCCCAGT
CACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGG
AGCTCCACCGCGGTGGCGGCCGCTCTAGCGATGAATTAGTTAAAGAACCAGACCAGTTAC
CACAAAAGGTTCAAGAAACAAGAAAACTCGTTAAAACAATACTAGACCAGGGTCATTTAT
CGCCATTTCTTGATTCCTTGCGCCCAATTTCATGGGATTTGGACCACACTTTGACACTTT
GCCCAATACCATCGACAATGGTTCTTTGCGACACTACTTCTGCACAATTTGATTTGACAT
ACAACGGTTGTAAAGTAATTAACCCTGGAAGTTTCATTCATAATAGACGTGCTAGGTATA
TGGAGTACGTTCCATCCTCAAAGAAAACTATACAAGAAGAGATATATATCTAAATAATGC
ATCAAAATAAGAACACTTAAACAAGCACGGTAGTGTTGGTACTCTGCCATCTATTTGTAT
GGATACTTTTTGAATTTTGATGTAACGGATTTCGGCATACAATATATGGGATCTATGGGG
ACTAAATTGTCGTATCCCATTAAACTTAAACCAGCAACACCAAAAACTGTATGGAAGACA
TCCACTTCATTTTCAGGCCTGTCGCTTATTCCACCCTTCTTCTCATCTTGACATTTGAGT
ATAAACTCAGTTAGCTTCTCATAATTTATCCAATCTAGTCTACCAATAATAGCTAATGAA
GATAAAACCCACCAACTATAGCAAACATCGGGCAGTTTACTTGGCCTACCGTTTAGTCCA
CCTTCTGGTAATTGTCGTTCGCAAAGCCACCACCCAATTTCTTCTAGTTGGTCGTCACTA
AGCATATCCAATTTGTTAGCAATTGCCAAAGCGCCAAGACAGGTAAAAGCTCTAGAACTA
GTGGATCCCCCGCAAACTTTGCTTGCCATTGTAGAGGCACACAATCCTGAGAATTTTTAG
ACAATGGGACAACGCTTTCTTCTTCCTCATCTGCGGGAGACCCCCATACAAACGCTTGGA
TCTTGGCAAGAGTCTTAGCAATATTGAATCCAAACGCGTGTTGTTGAGCCCTCTTTTTGG
AGATATTCTTGTAGTTTTTAGAACGTGTCCTTTGTGAAGCGTGTCTAACATGATTTCTTT
CGCGAGGCACAACCACCAATTTGCGCTCTTTTCGTATCCCCAATGTCTTTTCCTGAATTC
CCATTGTGATCTTCAATGCCAACCCAACCTTCTTTTTACTTTGTTGGGTCTAAATTATGT
TACTTTGTCCATTTCATCTCTTTTCAAGATGGAATCTAAAAGAAGAAAAACGACAAAAGG
AACAAAAGTGATGTGACGCGTTATTTTTCGCGTGACATAATAGAAAAATATTTCAACTGG
GCATCTTTAACACATGTTACAATAAAGTTGAGATGACTTGTTTATGCCTTTAAAGTTTGT
AAGTGGAACTAGAAGCGTCTATGTGTGAAATGTTTGGCTCTGGGAATGTTCTGCCTGTTA
AAATTCAGCCTCCGCTGCTTAGGCCACTGGCGTATAGAGTTTTATCAAGAAAATATGGTT
TATCAATTAAATCTGATGGACTGTCTGCTCTTGCAGAATTCGTTGGCACTAATATAGGTG
CTAACTGGAGACAAGGGCCTGCTACAATAAAATTTCTCGAGCAATTTGCTGCAGTATGGA
AACAACAAGAAAGAGGCCTTTTCATCGACCAGAGCGGGGTTAAAGAAGTTATACAGGAGA
TGAAAGAACGTGAGAAAGTCGAATGGAGTCATGAACACCCTATTCAACATGAGGAGAATA
TCTTGGGACGGACAGATGACGATGAGAACAACAGTGACGATGAAATGCCGATCGCTGCTG
ATTCATCCTTACAAAATGTTTCATTATCCTCACCCATGCGACAGCCCACCGAAAGAGATG
AATATAAACAGCCTTTCAAGCCAGAAAGCTCCAAGGCGTTGGACTGGAGGGACTACTTCA
AAGTTATCAATGCTTCTCAGCAACAAAGATTCTCATACAATCCGCACAAAATGCAATTTA
TTTTCGTCCCCAATAAAAAGCAGAATGGGCTAGGAGGCATTGCGGGTTTTCTACCAGATA
TAGAGGACAAAGTTCAAATGTTCTTGACAAGATACTACCTCACGAACGACAGAGTCATGA
GAAATGAAAACTTCCAAAACAGTGACATGTTTAATCCATTATCATCTATGGTGTCTTTAC
AAAATGAACTGTCCAATACTAACCGACAGCAGCAATCCAGCAGTATGAGTATCACCCCAA
TAAAAAACCTACTAGGTAGGGATGCTCAAAACTTTCTACTATGGGGGCTCTTAAATAAGA
ACTTTAAGGGCAATTGGTCACTGGAAGATCCGTCTGGATCTGTCGAAATTGACATTTCCC
AAACTATCCCTACACAAGGCCATTACTACGTGCCAGGTTGCATGGTTCTTGTGGAAGGAA
TATACTATTCTGTAGGAAATAAATTCCATGTTACCTCCATGACTTTACCCCCTGGTGAGA
GAAGAGAAATTACATTAGAAACGATAGGTAATCTCGATCTCTTAGGAATACATGGCATTT
CTAATAACAATTTCATTGCTCGTTTGGATAAAGATTTGAAGATTAGATTACACCTTTTGG
AGAAAGAATTAACAGATCATAAATTTGTAATTCTCGGCGCAAATTTGTTCCTAGATGATT
TGAAAATTATGACTGCACTCAGCAAAATTTTGCAAAAATTAAATGATGACCCACCGACCC
TATTAATTTGGCAAGGTTCTTTCACTTCAGTTCCCGTTTTCGCATCAATGAGTAGCCGGA
ATATAAGTAGTTCCACTCAATTTAAGAATAATTTCGATGCCTTGGCGACGCTTCTGTCAA
GATTTGACAATTTGACCGAAAATACCACAATGATATTTATTCCAGGTCCTAACGATTTAT
GGGGGTCGATGGTGTCATTGGGGGCAAGTGGGACATTACCGCAAGATCCAATTCCTAGTG
CGTTTACCAAAAAAATCAACAAGGTCTGTAAAAACGTTGTATGGAGCTCAAATCCAACTA
GAATAGCATACTTATCCCAAGAAATAGTCATTTTCAGGGACGATTTATCCGGAAGATTCA
AAAGACACCGTTTGGAATTCCCATTCAACGAGAGCGAAGATGTTTATACTGAAAACGATA
ACATGATGTCTAAAGACACCGATATTGTACCAATCGATGAATTAGTTAAAGAACCAGACC
AGTTACCACAAAAGGTTCAAGAAACAAGAAAACTCGTTAAAACAATACTAGACCAGGGTC
ATTTATCGCCATTTCTTGATTCCTTGCGCCCAATTTCATGGGATTTGGACCACACTTTGA
CACTTTGCCCAATACCATCGACAATGGTTCTTTGCGACACTACTTCTGCACAATTTGATT
TGACATACAACGGTTGTAAAGTAATTAACCCTGGAAGTTTCATTCATAATAGACGTGCTA
GGTATATGGAGTACGTTCCATCCTCAAAGAAAACTATACAAGAAGAGATATATATCTAAA
TAATGCATCAAAATAAGAACACTTAAACAAGCACGGTAGTGTTGGTACTCTGCCATCTAT
TTGTATGGATACTTTTTGAATTTTGATGTAACGGATTTCGGCATACAATATATGGGATCT
ATGGGGACTAAATTGTCGTATCCCATTAAACTTAAACCAGCAACACCAAAAACTGTATGG
AAGACATCCACTTCATTTTCAGGCCTGTCGCTTATTCCACCCTTCTTCTCATCTTGACAT
TTGAGTATAAACTCAGTTAGCTTCTCATAATTTATCCAATCTAGTCTACCAATAATAGCT
AATGAAGATAAAACCCACCAACTATAGCAAACATCGGGCAGTTTACTTGGCCTACCGTTT
AGTCCACCTTCTGGTAATTGTCGTTCGCAAAGCCACCACCCAATTTCTTCTAGTTGGTCG
TCACTAAGCATATCCAATTTGTTAGCAATTGCCAAAGCGCCAAGACAGGTAAAAGCTTGA
GCTGCATGGGATTCAGCATTAGGACATAATCCAAAGCCACCATCAAAATTATAACACTTG
AGTACAAAGTCTACAGCAGGGTCAACAACTTCAGACGTTAATTCACCCAAAATTGATAGT
GCACTCAAGGCTGTATAAACAAACCTTGTATCTACCTCCCCAAACCTGTCACCCTGGAAC
GAACCATCTTCCAATTGATTCCCACGGATGAAGGAAATCAAGCGAACCTTACGATCTTTT
CCAAGAACATCCAAAGCATCATAAGTGGCCAAGATCTGTACGGCGGACAATGTAGTTAAT
AAATGTGCATCATGTCTTGGAAATGGTGCGAAAGCGCCGTATTTGTCGTCCCAACAGCTC
AGAACAAATGATATAACCTCTTCTTTCACAAATGTCTCTGGAGAATCAAGCACACACAAG
CGTCTCGTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTCC
GAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT
TCCACACAACATAGGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAG
GTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG
CCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTC
TTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAA
CATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT
TTTCCATAGGCTCGGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTCCCCCCTGGAAGCTCCCTCGTGCG
CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAG
CGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTC
CAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA
CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCC
TAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC
CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGG
TTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTT
GATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGT
CATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAA
ATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGA
GGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTGCCCGTCGT
GTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCG
AGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGA
AGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGG
CATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATC
AAGGCGAGTTACATGATCCCCCATGTTGTGAAAAAAAGCGGTTAGCTCCTTCGGTCCTCC
GATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCA
TAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAAC
CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACG
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTC
GGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCG
TGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAAC
AGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT
ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA
CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA
AGTGCCACCTGACGTCTTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGG
CCCTTTCGTC
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Construction: |
Construction details described in details in Jaszczur et al. (2008) Genetics:633-647; the pKF117 plasmid constructed by Krzysztof Flis
|
Plasmid properties/Comments: |
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Plasmid map: |
|
Selectable Markers: |
AmpR, HIS3, URA3 |
Antibiotic concetration: |
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Plasmid size: |
|
Origin of replication/Incompatibility group: |
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Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
plasmid for integration of the mutated dpb2-GJ6 allele into DPB2 chromosomal locus; integration by transplacment |
Author: |
Malgorzata Jaszczur |
Availability: |
restricted |
Patent: |
|
Depositor: |
Malgorzata Jaszczur |
Institution/Department: |
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Deposition date: |
2009-03-21 17:53+01:00 |
References: |
Jaszczur et al. (2008) Genetics:633-647 |