| Base: |
Plasmid collection |
| Number in base: |
2547 |
| Plasmid name: |
pKF117dpb2-110 |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169 pKF117dpb2-110
|
| Plasmid Sequence: |
ND |
| Construction: |
Construction details described in details in Jaszczur et al. (2008) Genetics:633-647; the pKF117 plasmid constructed by Krzysztof Flis
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
AmpR, HIS3, URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
|
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid for integration of the mutated dpb2-110 allele into DPB2 chromosomal locus; integration by transplacment |
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Institution/Department: |
|
| Deposition date: |
2009-03-21 17:53+01:00 |
| References: |
Jaszczur et al. (2008) Genetics:633-647, Jaszczur et al. (2009) Mutation Research |