Base: |
Plasmid collection |
Number in base: |
2558 |
Plasmid name: |
pGJ104 |
Species: |
Escherichia coli |
Strain: |
DH5α |
Genotype: |
φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169 pGJ104
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Plasmid Sequence: |
AGCTTGCCGAGTTCTGAGATCACGCCCTTAAATTTAGCCGCCAAAACACCGGTCGAACGG
CGAAAATGGATCGGAGTCTTGATTGCACCCACACCTCGCATTTTGTGCACTAACATAAAA
TATGACGAAGTGAGAGTCATGACACACAGAATAACTTAAAAATACTTGAGGAAAAAGTTC
TCATATTATGAGGTCATAATAACTACATCGTTATTGTTGCGATATAACTTCAATGTTTTC
TGCCTGCAAAAGAATCCTTCCTTTTCCTTCTTTAATTGAAGAGTCCATGTAACCTTGTTT
CGTTACTTATATTTATTCACCTTTCTACTTTAGTCACATAACGTAATCTGGGCATAATCA
TTGAGGTATCATTATTAGCTTGAACTCGTGGTTCCTATTGTCGCCAGGTTTCTTTCAACG
CATTTGCTCTTTCCGCTTCGCATACGGCGGTGATCAAATGCAAAACTTACCCTCATCAGT
ATAAGAGAAGAAAAACTGCATGGACTATTCGCGTATTGCCTGGTAAAGCTATATAGTACT
ATTGGTAATGTTTGTACTGTTTTATGTACATGATGAATAAGAAGAGACAAATTTTACGTG
ATATATAAGTCTTCTATTTCTAGTATGGCAGGTATTTTATCAGTAGCATTAATATTACTA
TGAAGCCCTTTCAAGGAATAACATTTTGTCCGACTGCAATAAATAATGAAATCTTAGCTA
AAAAAATATCAAAGAAAATTATTAAGTTAGGAGGTATATTTTCTAAAGATTTGACCAGAC
AAGTTAATGTCCTTGTTGTTGGGTCCACCACCAATACAAACAAATTTAAATTTGCGGTGA
AGCATAGATTCGATATCATATTTATTGACATTCAAGCCATTGATGACATTTATCAGCTCT
GGCTGTCTGGAGAAAATATATTGCCGGACTCTAATACGGCAACAATGACAGGATCCACCT
ATGAGATGCTGAAGATACTTTACCGGCGATTTTCGTTTAAATATTTGCATAACTTCAATA
TCTTCATTGGCAGAATAACCGACACTAACATAACTTCCATCGATTCATTGGTTAGGAGTA
TTAAAAAGTTAGGGTGTTCTAGTTATAACTATCAAAACTTTGTAATCAAAGATACCTCTT
CACACAATGATGACGATGATCAAGGTCAGAATGGGCAGATATCCATTTTTGTTACGGATA
CTTTACTAGGAGCAAGAGTTAACGCTGCTATTGAACAAAATATACCTATTGTACATTTCA
AATGGATTCTCGATTGCCAAAAAAGAAGCGCACTGTTACCGTACGATCCCTATTATCTTT
TACCAAATATTAAAGACCTGCCCTATGATTCAATCGGATCAAACTCTTGCGATTGCTGGG
ATAAAATAAACACCACCTTTCCAACCAATATCGATGCCCAATCCTCTCTGCAGCGGCAGC
AATCCTCCAGTACCCTGACTCCTTCGCTCCCGAAAACATCATCCCTCTTGAATAAATTTA
AACCAAAGGGTGAAAAAATATGGGACAAGGCAATGTCTCTACAGCAGCATAGTAAGACAA
ATTTTTCAGTACTAGGGCAGTCACCATTATCGATAAATAACAAACAAGAAGATTTAAGTG
ATAATAGTACTTTGATTTTCAAAAATTGTGCGTTTATCATTCATCATATTTTTCCTGGCA
ATCACCGTTCAATACTAACCAAAATTGTTGTTCAAAATGGGGGTAAAATTGAGACGTCTT
ACTTGAGCGGCATTTACGATCATTCTTATTACATCATTCCAAGTAATAAAGCTCTTGACT
CCTTCAATGATTTACCTGAGATTATAGATGATAATGATGGTATAGTTACAGAATTTTTCA
TTGAACGCTGCTTGTATTATCAAAAATTACTACACCCAATAGATTTATGGTCAAAACCCT
TCCTCAGCACAATAGAGTTTCAAGTTTCGTCTTCTTCAAAGTTATTGCATCATGAATTTT
CTTCTTCCCCTTTTCTGAATGTTACTATCACTGGATTCTCTGGCGTAGAGCTGTTACATC
TGACTAAAGTATTAAATCTTCTAAAACCAATGGGCATCAATTATGTAGAATACCTCAATA
AATCCACTGACATTCTGCTAATCAACTTAGCAGCTTTACCCAGTATCCCGAAAACCCATC
CGTTATGGTCGAATGAATTTAGCGATCTTTTTACTCAGTTTTGCATTAATAACAATAATG
ATGATCCTGGTGATAATAACAGAAAAGATTTTCAAAATAATTCAATCTTGAGAAATTCGA
TGAAAAGGAAAATTGAATATATCAAGAAATTCCACTCCATACCGGTAGTTACTCCAGCAT
TTATTTTTAAATTATTGTCCGCTGCATCTGGAGAAAATAATGAAATCTTTTTAAACAATA
TCAAGTGGTGTATTATCTGCCCAAGAGGACACAAGGACGATTTTAAATGTAAGATAAAAA
AACCATACTATACCAGCATTAGTTCAGAAAAAAAGTACCAAAACAATGATCCAAAAATCG
ACAAAACTATTCTTTTGAAAAGAAACAATTCCTCATTATCGGAGCACTCTATGAAAGATA
CCAAAAACGAATTATTGCAGAAAATTAGAGAAACTGATTCTGGAAGAAAAAAGCGTAGTG
TCTCATCGAGTATCATGGATGTTTCTTCAGAGAGACAAATGCCGGATACGAAAAGGATCA
AGTTGGAGTCACTGCCAAAAAATTTCGTTCCTAAACAAATTAAACGAACCACGAGTTGGG
GCACAATAATGTCAGAAAATGTGCCTACAGAGCAGCCGACTGCAATTTCTAATCCAGAAG
AGATCCCAAGAACTGAGGAAGTTTCACATACTCAAGTTACCTATGGCTCCATTCAAGATA
AGAAACGTACTGCCTCTTTAGAAAAACCTATGAGACGACAGACAAGAAATCAGACAAAGG
AATTAGATTCTTGAAATGTAGTCCGTAATTTTATAAGATATTCATTTACATACGCCATCT
ACAGCATTATTCAAATCTACTCATCTATATGTATTACCGTTTTGTATGATAATACTTTCC
ATGACATGCTCGCGTGAAAAAACAGCATGAGAAAAAGAGGATCGCAATAAGAAGACACGT
AAATATCTAAATAAATGGCAACAGTACATCAGAAGAATATGTCGACCTCGAGGGGGGGCC
CGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTAC
AACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCC
CTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGC
GCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTG
TGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCG
CTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGG
GGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATT
AGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGT
TGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTA
TCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAA
ATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT
CCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATA
ACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATA
ATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTC
TGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAA
TAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATG
CGTCTCCCTTGTCATCTAAACCCACACCGGGTGTCATAATCAACCAATCGTAACCTTCAT
CTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCG
CAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATT
CTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAAC
CGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTA
TTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTC
TGTCTTCGAAGAGTAAAAAATTGTACTTGGCGGATAATGCCTTTAGCGGCTTAACTGTGC
CCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGAC
CTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACA
AGTTTGTTTGCTTTTCGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAGGATGAG
TAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTT
TTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATA
TGCGTATATATACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGA
TTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATG
ATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCG
CATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTC
TGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGA
GGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTT
TATAGGTTAATGTCATGATAATAATGGTTTCTTAGTATGATCCAATATCAAAGGAAATGA
TAGCATTGAAGGATGAGACTAATCCAATTGAGGAGTGGCAGCATATAGAACAGCTAAAGG
GTAGTGCTGAAGGAAGCATACGATACCCCGCATGGAATGGGATAATATCACAGGAGGTAC
TAGACTACCTTTCATCCTACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACAC
GCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGA
CGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCG
GAAACGCTTTGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCAG
AGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACC
GGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGT
ATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGC
TCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACT
CTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAA
ATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTT
TCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCA
CATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCG
CTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGAC
ACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACT
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAG
CGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAAT
AGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAA
ATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGT
AAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTT
GAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGT
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATA
CTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTT
GATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCC
GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTG
CAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACT
CTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTG
TAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTG
CTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAC
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACA
CAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGA
GAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTC
GGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCT
GTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGG
AGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCT
TTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCC
TTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGC
GAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCAT
TAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATT
AATGTGAGTTACCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCCT
ATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGAT
TACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGT
GGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCA
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Construction: |
the DPB11 containing fragment was cloned into pRS426 plasmid; the DPB11 containing fragment was PCR amplified using pair of primers:
1-DPB11 5' GCGAAGCTTGCCGAGTTCTGAGATCAC and 2_dpb11 5' GGGGTCGACATATTCTTCTGATGTAC 3' than it was HindII/SalI digested and ligated with HindIII/SalI-pRS426 |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
AmpR |
Antibiotic concetration: |
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Plasmid size: |
8872 bp |
Origin of replication/Incompatibility group: |
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Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
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Author: |
Malgorzata Jaszczur |
Availability: |
restricted |
Patent: |
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Depositor: |
Malgorzata Jaszczur |
Institution/Department: |
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Deposition date: |
2009-03-21 17:53+01:00 |
References: |
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