Base: |
Plasmid collection |
Number in base: |
2562 |
Plasmid name: |
pIL253_S7 |
Species: |
Lactococcus lactis |
Strain: |
IL1403ΔhsdS |
Genotype: |
ΔhsdS |
Plasmid Sequence: |
GATCCCAAGAAAGTGGTGGGGACTATGGCGAAAATAGATGATTCAGTTAAAAAGAAAGTT
CCTGAATTGCGATTTAAAGGATTTACGAATGATTGGGAAGAGCGTAAGTTAGGAGAATTA
TCTAATATTGTTGGTGGTGGAACACCAAGTACATCGAACCCTGAATACTGGGACGGTGAT
ATTGATTGGTATGCACCAGCTGAAATTGGAGAACAAAGTTATGTTAGCAAAAGTAAAAAG
ACTATTACTGAACTAGGTCTAAAGAAGAGTTCAGCTCGAATTTTACCAGTAGGAACCGTC
TTATTTACTTCTCGTGCTGGTATCGGAAACACCGCTATATTAGCCAAAGAAGCTACAACT
AATCAAGGGTTTCAATCAATTGTTCCTGATCAAAATAAACTTGATAGTTATTTTATTTTT
TCAAGAACTAATGAGCTCAAGCGATATGGGGAAGTCACTGGTGCAGGATCTACTTTTGTT
GAGGTTTCAGGTAAGCAAATGTCGAAAATGTCGATTATGGTACCTGAACTTTCAGAACAA
CAAAAAATTGGAAACTTCTTTAAAGAGTTAGACAACACTATCGCTCTTCATCAGCGTAAG
TTAGATTTGTTGAAAGAACAGAAAAAAGGCTACTTGCAAAAAATGTTCCCTAAAAATGGT
GCCAAAGTTCCTGAATTGCGATTTGCGGGGTTTGCTGACGATTGGGAAGAGCGTAAGTTA
GGAGATATTACTAAAATAAGCACCGGAAAATTAGATGCAAATGCAATGGTTGAAAATGGC
AAGTATGATTTTTATACTTCTGGAATTAAAAAATATAGGATCGATGTAGCAGCGTTTGAA
GGACCGTCCATTACAATTGCAGGTAATGGAGCAACAGTTGGATACATGCATTTAGCGGAT
AATAAGTTCAATGCCTATCAACGAACTTATGTGCTCCAAGAGTTTCTAGTAGATAGAAGT
TTTATATTTTCAGAAATTGGAAATAAGCTGCCTAAAAAAATAAAACAGGAAGCTCGTACA
GGAAATATTCCGTACATTGTTATGGACATGTTGACTGAACTGAAGCTATCGATTCCTCAG
AATAATTCAGAGCAACAAAAAATAGGTTCATTCTTTAAACAGCTAGATGACACTATCGCT
CTTCATCAACGTAAGTTAGATTTGTTGAAAGAACAGAAAAAAGGCTTTTTACAAAAGATG
TTTGTTTAGGGTCTATAATTAGATAATAACCCCCTGCAGCCAAGCTTATCGATTCTAGAC
TCGAGAGCTCTCGAGTCTAGAATCGATACGATTTTGAAGTGGCAACAGATAAAAAAAAGC
AGTTTAAAATTGTTGCTGAACTTTTAAAACAAGCAAATACAATCATTGTCGCAACAGATA
GCGACAGAGAAGGCGAAAACATTGCCTGGTCGATCATTCATAAAGCAAATGCCTTTTCTA
AAGATAAAACGTATAAAAGACTATGGATCAATAGTTTAGAAAAAGATGTGATCCGTAGCG
GTTTTCAAAATTTGCAACCAGGAATGAATTACTATCCCTTTTATCAAGAAGCGCAAAAGA
AAAACGAAATGATACACCAATCAGTGCAAAAAAAGATATAATGGGAGATAAGACGGTTCG
TGTTCGTGCTGACTTGCACCATATCATAAAAATCGAAACAGCAAAGAATGGCGGAAACGT
AAAAGAAGTTATGGAAATAAGACTTAGAAGCAAACTTAAGAGTGTGTTGATAGTGCAGTA
TCTTAAAATTTTGTATAATAGGAATTGAAGTTAAATTAGATGCTAAAAATTTGTAATTAA
GAAGGAGTGATTACATGAACAAAAATATAAAATATTCTCAAAACTTTTTAACGAGTGAAA
AAGTACTCAACCAAATAATAAAACAATTGAATTTAAAAGAAACCGATACCGTTTACGAAA
TTGGAACAGGTAAAGGGCATTTAACGACGAAACTGGCTAAAATAAGTAAACAGGTAACGT
CTATTGAATTAGACAGTCATCTATTCAACTTATCGTCAGAAAAATTAAAACTGAATACTC
GTGTCACTTTAATTCACCAAGATATTCTACAGTTTCAATTCCCTAACAAACAGAGGTATA
AAATTGTTGGGAGTATTCCTTACCATTTAAGCACACAAATTATTAAAAAAGTGGTTTTTG
AAAGCCATGCGTCTGACATCTATCTGATTGTTGAAGAAGGATTCTACAAGCGTACCTTGG
ATATTCACCGAACACTAGGGTTGCTCTTGCACACTCAAGTCTCGATTCAGCAATTGCTTA
AGCTGCCAGCGGAATGCTTTCATCCTAAACCAAAAGTAAACAGTGTCTTAATAAAACTTA
CCCGCCATACCACAGATGTTCCAGATAAATATTGGAAGCTATATACGTACTTTGTTTCAA
AATGGGTCAATCGAGAATATCGTCAACTGTTTACTAAAAATCAGTTTCATCAAGCAATGA
AACACGCCAAAGTAAACAATTTAAGTACCGTTACTTATGAGCAAGTATTGTCTATTTTTA
ATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTGTAAATTTGGA
AAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGGTATACTACTGACAGCTTCC
AAGGAGCTAAAGAGGTCCCTAGCGCTCTTATCATGGGGAAGCTCGGATCATATGCAAGAC
AAAATAAACTCGCAACAGCACTTGGAGAAATGGGACGAATCGAGAAAACCCTCTTTACGC
TGGATTACATATCTAATAAAGCCGTAAGGAGACGGGTTCAAAAAGGTTTAAATAAAGGAG
AAGCAATCAATGCATTAGCTAGAACTATATTTTTTGGACAACGTGGAGAATTTAGAGAAC
GTGCTCTCCAAGACCAGTTACAAAGAGCTAGTGCACTAAACATAATTATTAACGCTATAA
GTGTGTGGAACACTGTATATATGGAAAAAGCCGTAGAAGAATTAAAAGCAAGAGGAGAAT
TTAGAGAAGATTTAATGCCATATGCGTGGCCGTTAGGATGGGAACATATCAATTTTCTTG
GAGAATACAAATTTGAAGGATTACATGACACTGGGCAAATGAATTTACGTCCTTTACGTA
TAAAAGAGCCGTTTTATTCTTAATATAACGGCTCTTTTTATAGAAAAAATCCTTAGCGTG
GTTTTTTTCCGAAATGCTGGCGGTACCCCAAGAATTAGAAATGAGTAGATCAAATTATTC
ACGAATAGAATCAGGAAAATCAGATCCAACCATAAAAACACTAGAACAAATTGCAAAGTT
AACTAACTCAACGCTAGTAGTGGATTTAATCCCAAATGAGCCAACAGAACCAGAGCCAGA
AACAGAATCAGAACAAGTAACATTGGATTTAGAAATGGAAGAAGAAAAAAGCAATGACTT
CGTGTGAATAATGCACGAAATCGTTGCTTATTTTTTTTTAAAAGCGGTATACTAGATATA
ACGAAACAACGAACTGAATAGAAACGAAAAAAGAGCCATGACACATTTATAAAATGTTTG
ACGACATTTTATAAATGCATAGCCCGATAAGATTGCCAAACCAACGCTTATCAGTTAGTC
AGATGAACTCTTCCCTCGTAAGAAGTTATTTAATTAACTTTGTTTGAAGACGGTATATAA
CCGTACTATCATTATATAGGGAAATCAGAGAGTTTTCAAGTATCTAAGCTACTGAATTTA
AGAATTGTTAAGCAATCAATCGGAAATCGTTTGATTGCTTTTTTTGTATTCATTTATAGA
AGGTGGAGTTTGTATGAATCATGATGAATGTAAAACTTATATAAAAAATAGTTTATTGGA
GATAAGAAAATTAGCAAATATCTATACACTAGAAACGTTTAAGAAAGAGTTAGAAAAGAG
AAATATCTACTTAGAAACAAAATCAGATAAGTATTTTTCTTCGGAGGGGGAAGATTATAT
ATATAAGTTAATAGAAAATAACAAAATAATTTATTCGATTAGTGGAAAAAAATTGACTTA
TAAAGGAAAAAAATCTTTTTCAAAACATGCAATATTGAAACAGTTGAATGAAAAAGCAAA
CCAAGTTAATTAAACAACCTATTTTATAGGATTTATAGGAAAGGAGAACAGCTGAATGAA
TATCCCTTTTGTTGTAGAAACTGTGCTTCATGACGGCTTGTTAAAGTACAAATTTAAAAA
TAGTAAAATTCGCTCAATCACTACCAAGCCAGGTAAAAGCAAAGGGGCTATTTTTGCGTA
TCGCTCAAAATCAAGCATGATTGGCGGTCGTGGTGTTGTTCTGACTTCCGAGGAAGCGAT
TCAAGAAAATCAAGATACATTTACACATTGGACACCCAACGTTTATCGTTATGGAACGTA
TGCAGACGAAAACCGTTCATACACGAAAGGACATTCTGAAAACAATTTAAGACAAATCAA
TACCTTCTTTATTGATTTTGATATTCACACGGCAAAAGAAACTATTTCAGCAAGCGATAT
TTTAACAACCGCTATTGATTTAGGTTTTATGCCTACTATGATTATCAAATCTGATAAAGG
TTATCAAGCATATTTTGTTTTAGAAACGCCAGTCTATGTGACTTCAAAATCAGAATTTAA
ATCTGTCAAAGCAGCCAAAATAATTTCGCAAAATATCCGAGAATATTTTGGAAAGTCTTT
GCCAGTTGATCTAACGTGTAATCATTTTGGTATTGCTCGCATACCAAGAACGGACAATGT
AGAATTTTTTGATCCTAATTACCGTTATTCTTTCAAAGAATGGCAAGATTGGTCTTTCAA
ACAAACAGATAATAAGGGCTTTACTCGTTCAAGTCTAACGGTTTTAAGCGGTACAGAAGG
CAAAAAACAAGTAGATGAACCCTGGTTTAATCTCTTATTGCACGAAACGAAATTTTCAGG
AGAAAAGGGTTTAATAGGGCGTAATAACGTCATGTTTACCCTCTCTTTAGCCTACTTTAG
TTCAGGCTATTCAATCGAAACGTGCGAATATAATATGTTTGAGTTTAATAATCGATTAGA
TCAACCCTTAGAAGAAAAAGAAGTAATCAAAATTGTTAGAAGTGCCTATTCAGAAAACTA
TCAAGGGGCTAATAGGGAATACATTACCATTCTTTGCAAAGCTTGGGTATCAAGTGATTT
AACCAGTAAAGATTTATTTGTCCGTCAAGGGTGGTTTAAATTCAAGAAAAAAAGAAGCGA
ACGTCAACGTGTTCATTTGTCAGAATGGAAAGAAGATTTAATGGCTTATATTAGCGAAAA
AAGCGATGTATACAAGCCTTATTTAGTGACGACCAAAAAAGAGATTAGAGAAGTGCTAGG
CATTCCTGAACGGACATTAGATAAATTGCTGAAGGTACTGAAGGCGAATCAGGAAATTTT
CTTTAAGATTAAACCAGGAAGAAATGGTGGCATTCAACTTGCTAGTGTTAAATCATTGTT
GCTATCGATCATTAAAGTAAAAAAAGAAGAAAAAGAAAGCTATATAAAGGCGCTGACAAA
TTCTTTTGACTTAGAGCATACATTCATTCAAGAGACTTTAAACAAGCTAGCAGAACGCCC
TAAAACGGACACACAACTCGATTTGTTTAGCTATGATACAGGCTGAAAATAAAACCCGCA
CTATGCCATTACATTTATATCTATGATACGTGTTTGTTTTTTCTTTGCTGTTTAGCGAAT
GATTAGCAGAAATATACAGAGTAAGATTTTAATTAATTATTAGGGGGAGAAGGAGAGAGT
AGCCCGAAAACTTTTAGTTGGCTTGGACTGAACGAAGTGAGGGAAAGGCTACTAAAACGT
CGAGGGGCAGTGAGAGCGAAGCGAACACTTGATTTTTTAATTTTCTATCTTTTATAGGTC
ATTAGAGTATACTTATTTGTCCTATAAACTATTTAGCAGCATAATAGATTTATTGAATAG
GTCATTTAAGTTGAGCATATTAGAGGAGGAAAATCTTGGAGAAATATTTGAAGAACCCGA
TTACATGGATTGGATTAGTTCTTGTGGTTACGTGGTTTTTAACTAAAAGTAGTGAATTTT
TGATTTTTGGTGTGTGTGTCTTGTTGTTAGTATTTGCTAGTCAAAGTGATTAAATAGAAT
TCTCATGTTTGACAGCTTATCATCGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTC
CCGGG
|
Construction: |
Insert (hsdS gene of pIL7) was generated by PCR and inserted into pIL253 vector
|
Plasmid properties/Comments: |
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Plasmid map: |
|
Selectable Markers: |
Ery |
Antibiotic concetration: |
|
Plasmid size: |
6185bp |
Origin of replication/Incompatibility group: |
pIL253, Simon D, Chopin A. 1988. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie. 70(4):559-66. |
Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
this strain is 350- and 22-fold more resistant to phages bIL170 and bIL67 in comparison to the plasmid-free IL1403 strain |
Author: |
Roman Gorecki |
Availability: |
restricted |
Patent: |
|
Depositor: |
Roman Gorecki |
Institution/Department: |
|
Deposition date: |
2010-09-02 12:44+01:00 |
References: |
R. Górecki, PhD thesis |