| Base: |
Plasmid collection |
| Number in base: |
26 |
| Plasmid name: |
pOU82Ω-ε-ζ |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(φ80dlacZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk-mk+)supE44relA1deoR Δ(lacZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
2,2kb BamHI-PstI fragment of pBT286 (derivative of pSM19035 from S.pyogenes,containing Δ, Ω,epsilon and zeta genes) subcloned into pMTL22, and inserted as EcoRI-BamHI fragment into EcoRI-BamHI sites of pOU82 vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
14,8kb |
| Origin of replication/Incompatibility group: |
R1 |
| Host range: |
- |
| Other genetic elements: |
lacAYZ, cI857, |
| Strain properties/Comments: |
replication dependent on increasing temperature |
| Author: |
U. Zielenkiewicz |
| Availability: |
restricted |
| Patent: |
- |
| Depositor: |
U. Zielenkiewicz |
| Institution/Department: |
|
| Deposition date: |
16.09.2002 |
| References: |
Jensen et al., 1995. Mol.Microbiol.17:211-220 |