Base: |
Plasmid collection |
Number in base: |
2650 |
Plasmid name: |
pAMB7.5 |
Species: |
E. coli |
Strain: |
DH5α |
Genotype: |
F-(Φ80d lacZ ΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1 deoR ΔlacZYA-argF) U196
|
Plasmid Sequence: |
unaviable |
Construction: |
286 nt PCR fragment amplified on miniRA3* containing ‘repA-repBp(repXp-repX) from pRA3 cloned into pPT01 vector as the BamHI-SphI fragment to obtain repXp-repX –xylE transcriptional fusion (RA3 coordinates 45619-45905) |
Plasmid properties/Comments: |
|
Plasmid map: |
|
Selectable Markers: |
kan |
Antibiotic concetration: |
|
Plasmid size: |
11 kb |
Origin of replication/Incompatibility group: |
oriSC101
|
Host range: |
|
Other genetic elements: |
|
Strain properties/Comments: |
|
Author: |
Aleksandra Markowska |
Availability: |
restricted |
Patent: |
|
Depositor: |
Aleksandra Markowska |
Institution/Department: |
Zak?ad Biochemii Drobnoustrojów |
Deposition date: |
25.08.2014 |
References: |
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