| Base: |
Plasmid collection |
| Number in base: |
2656 |
| Plasmid name: |
pAMB7.14 |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
F-(Φ80d lacZ ΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1 deoR ΔlacZYA-argF) U196
|
| Plasmid Sequence: |
unaviable |
| Construction: |
376 nt PCR fragment amplified on miniRA3* containing repAp-repA-1 from pRA3 (mutation A→C in repA) cloned into pPT01 vector as the BamHI fragment to obtain repAp-repA-1 –xylE transcriptional fusion (RA3 coordinates 45365-45741)
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
11.1 kb |
| Origin of replication/Incompatibility group: |
oriSC101
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aleksandra Markowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aleksandra Markowska |
| Institution/Department: |
Zak?ad Biochemii Drobnoustrojów |
| Deposition date: |
25.08.2014 |
| References: |
|