| Base: |
Plasmid collection |
| Number in base: |
2672 |
| Plasmid name: |
pAMB8.32 |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
F-(Φ80d lacZ ΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1 deoR ΔlacZYA-argF) U196
|
| Plasmid Sequence: |
unaviable |
| Construction: |
fragment around 2 kb containing repAp-repA-1-repBp(repXp-repX)- 565 nt 5’ end of repB from pRA3 with mutation A→C in repA cloned into pBBR1MCS-3 vector as the EcoRI-KpnI fragment
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
tet |
| Antibiotic concetration: |
|
| Plasmid size: |
7.2 kb |
| Origin of replication/Incompatibility group: |
IncA/C
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aleksandra Markowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aleksandra Markowska |
| Institution/Department: |
Zak?ad Biochemii Drobnoustrojów |
| Deposition date: |
26.08.2014 |
| References: |
|