| Base: |
Plasmid collection |
| Number in base: |
2675 |
| Plasmid name: |
pAMB10.33 |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
F-(Φ80d lacZ ΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1 deoR ΔlacZYA-argF) U196
|
| Plasmid Sequence: |
unaviable |
| Construction: |
T7p– repB –His6; 1377 nt PCR fragment containing repBRA3 (RA3 coordinates 1-1377); cloned into pET22b as the EcoRI-HindIII fragment |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
6.9 kb |
| Origin of replication/Incompatibility group: |
oriMB1
Aleksandra Markowska
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Aleksandra Markowska |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Aleksandra Markowska |
| Institution/Department: |
Zak?ad Biochemii Drobnoustrojów |
| Deposition date: |
26.08.2014 |
| References: |
|