| Base: |
Plasmid collection |
| Number in base: |
295 |
| Plasmid name: |
pBT205copS |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(f80 lacZΔM15) recA1endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
674bp fragment (copS promoter) was cut out of plasmid pBT366 by AccI (position 18560 of pDB101,blunted by T4 polymerase) and HindIII. Fragment was ligated into pBT205 vector cut by EcoRI, blunted by T4 polymerase and digested by HindIII |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, cam |
| Antibiotic concetration: |
|
| Plasmid size: |
about 11kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Piotr Ceglowski |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz PhD thesis |