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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 300
Plasmid name: pRC1
Species: Bacillus subtilis
Strain: YB886
Genotype: amyE metB5 trpC2 xin-1 attSPbβ
Plasmid Sequence:
Construction: pDB101 derivative, carrying copS with its promoter, repS and ery genes. Blunted by T4 polymerase fragment EcoRI-FspI (positions 16738 and 2546 of pDB101) was ligated with HindIII-HindIII (blunted) fragment encompassing erm1 and erm2 genes (7824–9622)
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: ery
Antibiotic concetration:
Plasmid size: 6831bp
Origin of replication/Incompatibility group: inc18
Host range: Gram positive bacteria
Other genetic elements:
Strain properties/Comments: pSm19035 derivative
Author: I. Sitkiewicz
Availability: restricted
Patent:
Depositor: I. Sitkiewicz
Institution/Department:
Deposition date: 06.08.2002
References: I. Sitkiewicz, PhD thesis