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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 36
Plasmid name: pBT346-8m
Species: Escherichia coli
Strain: DH5α
Genotype: F-(φ80dlacZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk-mk+)supE44relA1deoR Δ(lacZYA-argF)U169
Plasmid Sequence:
Construction: 1,3kb PCR product corresponding to epsilon -zeta genes of pSM19035 plasmid (S. pyogenes) inserted into PstI-BamHI sites of pHP13 vector. Zeta gene contains two point mutations (A7089T, G7184A) and is inactive.
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: ery cam
Antibiotic concetration:
Plasmid size: 6,2kb
Origin of replication/Incompatibility group: pBR322, pTA1060
Host range: E.coli, B.subtilis
Other genetic elements:
Strain properties/Comments:
Author: R.Pankiewicz
Availability: restricted
Patent:
Depositor: U. Zielenkiewicz
Institution/Department:
Deposition date: 16.09.2002
References: