α β γ δ ε ζ η θ ι κ λ μ ν ξ ο π ρ Σ σ τ υ φ χ ψ ω Δ Γ Ω Σ Λ Ψ Φ Ξ Θ Π °C Greek letters

Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 45
Plasmid name: pGAD424-Δ3ε
Species: Escherichia coli
Strain: DH5α
Genotype: F-(f80dlacZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk-mk+)supE44relA1deoR Δ(lacZYA-argF)U169
Plasmid Sequence:
Construction: 255bp PCR product corresponding to 5C-terminal amino acids deletion of epsilon gene (of pSM19035 plasmid from S.pyogenes) inserted into EcoRI-BamHI sites of pGAD424 vector (two-hybrid system)
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp
Antibiotic concetration:
Plasmid size: 6,91kb
Origin of replication/Incompatibility group:
Host range: E. coli; S. cerevisiae
Other genetic elements:
Strain properties/Comments:
Author: U.Zielenkiewicz
Availability: restricted
Patent: -
Depositor: U. Zielenkiewicz
Institution/Department:
Deposition date: 16.09.2002
References: Zielenkiewicz, U. Ph.D. thesis: Role of genes epsilon and zeta of plasmid pSM19035 in its stable maintenance in bacterial cells. Warszawa, 2001.