Base: |
Plasmid collection |
Number in base: |
451 |
Plasmid name: |
pGAD424-APN1 |
Species: |
Escherichia coli |
Strain: |
DH5 α |
Genotype: |
supE44 Δ lacU169 (FI 80 lacZ Δ M15) hdsR17 recA1 endA1 gyrA96 thi-1 relA1 |
Plasmid Sequence: |
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Construction: |
1,4 kb PCR fragment of APN1 with EcoRI place in the upper primer was digested with EcoRI, ligated with pUC19 EcoRI-SmaI. EcoRI-BamHI fragment was ligated with pGAD424 digested by EcoRI-BamHI |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
amp leu |
Antibiotic concetration: |
|
Plasmid size: |
8,1 kb |
Origin of replication/Incompatibility group: |
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Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
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Author: |
Justyna McIntyre |
Availability: |
restricted |
Patent: |
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Depositor: |
A. Skoneczna |
Institution/Department: |
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Deposition date: |
01.02.2003 |
References: |
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