| Base: |
Plasmid collection |
| Number in base: |
461 |
| Plasmid name: |
pPM70 |
| Species: |
Escherichia coli |
| Strain: |
DH5 α |
| Genotype: |
supE44 Δ lacU169 (FI 80 lacZ Δ M15) hdsR17 recA1 endA1 gyrA96 thi-1 relA1 |
| Plasmid Sequence: |
|
| Construction: |
SpeI-EcoIRCRI pGEM5 blunted by Klenow was religated to loose PstI site. Than digested by SmaI, ligated with PCR fragment of UMP1. The PstI-ClaI part was remooved. The rest was ligated with PstI-EcoICRI fragment of pFA6A harbouring G418 resistance gene. |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
4,3 kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Piotr Mieczkowski |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
A. Skoneczna |
| Institution/Department: |
|
| Deposition date: |
01.02.2003 |
| References: |
|