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Central Collection of Strains (IBB)
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Base:
Plasmid collection
Number in base:
463
Plasmid name:
pRAD30::LEU2
Species:
Escherichia coli
Strain:
DH5α
Genotype:
φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169
Plasmid Sequence:
Construction:
1,6 kb fragment AccI-HpaI from pRS315 carring LEU2 gene was ligated with 4322 StuI and BglII fragment from pJM2 blunted by Klenow. Source of gene blaster RAD30::LEU2
Plasmid properties/Comments:
Plasmid map:
Selectable Markers:
Amp
Antibiotic concetration:
Plasmid size:
5,9 kb
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author:
Adrianna Skoneczna
Availability:
restricted
Patent:
Depositor:
A. Skoneczna
Institution/Department:
Deposition date:
2003-06-16
References:
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