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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 463
Plasmid name: pRAD30::LEU2
Species: Escherichia coli
Strain: DH5α
Genotype: φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169
Plasmid Sequence:
Construction: 1,6 kb fragment AccI-HpaI from pRS315 carring LEU2 gene was ligated with 4322 StuI and BglII fragment from pJM2 blunted by Klenow. Source of gene blaster RAD30::LEU2
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: Amp
Antibiotic concetration:
Plasmid size: 5,9 kb
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Adrianna Skoneczna
Availability: restricted
Patent:
Depositor: A. Skoneczna
Institution/Department:
Deposition date: 2003-06-16
References: