| Base: |
Plasmid collection |
| Number in base: |
499 |
| Plasmid name: |
pGB2ts |
| Species: |
E.coli |
| Strain: |
DJ125 (EC5003) |
| Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 recA56 |
| Plasmid Sequence: |
|
| Construction: |
derivative of pGB2 (mini pSC101 SpR/StrR) containing repA gene with point mutation causing that the replication protein is temperature sensitive |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Spc/Str |
| Antibiotic concetration: |
|
| Plasmid size: |
4025 bp |
| Origin of replication/Incompatibility group: |
pSC101 origin |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
the concentration of spc depends on quality of antibiotic (20-40 ug/ml in LB) |
| Author: |
Michael Clerget |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
Clerget, M.(1991). New Biol. 3, 780-788. |