Base: |
Plasmid collection |
Number in base: |
500 |
Plasmid name: |
pMLO6 |
Species: |
E.coli |
Strain: |
DJ125 (EC5004 |
Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 recA56 |
Plasmid Sequence: |
|
Construction: |
derivative of pGB2ts containing the parS site of P1 plasmid cloned into EcoRI site |
Plasmid properties/Comments: |
|
Plasmid map: |
|
Selectable Markers: |
Spc / Str |
Antibiotic concetration: |
|
Plasmid size: |
4225 bp |
Origin of replication/Incompatibility group: |
pSC101 origin |
Host range: |
|
Other genetic elements: |
|
Strain properties/Comments: |
the concentration of spc depends on the quality of antibiotic (20-40 ug/ml in LB) |
Author: |
Malgorzata Lobocka |
Availability: |
non restricted |
Patent: |
|
Depositor: |
O.Juszczuk |
Institution/Department: |
|
Deposition date: |
2004-05-06 |
References: |
Lobocka, M. and Yarmolinsky, M. (1996). J. Mol. Biol. 259: 366-382.501P |