| Base: |
Plasmid collection |
| Number in base: |
528 |
| Plasmid name: |
pZC84 |
| Species: |
E.coli |
| Strain: |
DPB699 (EC5253) |
| Genotype: |
thi1 thr1 deoB deoC fhuA21 recA56 supE44 |
| Plasmid Sequence: |
|
| Construction: |
4132 bp NheI-EcoRI fragment of pBR322 was ligated with 1122 bp NdeI-SpeI fragment of pPM30 carrying the wild-type repA of pSC101; then 1323 bp HindIII-SmaI fragment of Tn5 carrying gene conferring kan resistance was inserted in ScaI site of pBR322 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
6.58 kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
- |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
Biek, D. P. and Cohen, S. N. (1989). J. Bacteriol. 171: 2066-2074 |