| Base: |
Plasmid collection |
| Number in base: |
531 |
| Plasmid name: |
pOBK15 |
| Species: |
E.coli |
| Strain: |
DH5 α (EC5269) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
isogenic repA- control for pZC84; 5263 bp SspI-BamHI fragment of pZC84 containing kan resistance gene was ligated with 336 bp SspI-BamHI fragment of pBR322 early depleted of EcoRI-NheI fragment containing the part of tet gene |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
5699 bp |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|