| Base: |
Plasmid collection |
| Number in base: |
533 |
| Plasmid name: |
pOBK45 |
| Species: |
E.coli |
| Strain: |
DH5 α (EC5375) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
pUC18-based plasmid containing parS of P1 and the replication origin with ca. 500 bp repAts gene of pSC101 from pMLO60, 1454 bp SmaI-AflIII fragment of pMLO60 repaired at AflIII end was inserted in HincII site of pUC18 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
4140 bp |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid contains two origins of replication: pUC18 and pSC101but replicates only from pUC18 |
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
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