| Base: |
Plasmid collection |
| Number in base: |
534 |
| Plasmid name: |
pOBK46 |
| Species: |
E.coli |
| Strain: |
DH5 α (EC5376) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
pOBK45-derivative containing 5’ moiety of repAts of pSC101, 220 bp SpeI-HindIII fragment of PCR product obtained by amplification of 5’ end of repAts with primers OMLO182 and OMLO183 was inserted in place of small SpeI-HindIII fragment of pOBK45 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
3866 bp |
| Origin of replication/Incompatibility group: |
ori pUC18 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid contains two origins of replication: pUC18 and pSC101but replicates only from pUC18 |
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|