| Base: |
Plasmid collection |
| Number in base: |
535 |
| Plasmid name: |
pOBK49 |
| Species: |
E.coli |
| Strain: |
DH5 α (EC5381 |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
pSR-based plasmid containing 5’ fragment of repAts and replication origin of pSC101 in close vicinity to the P1 centromeric site, XbaI-HindIII fragment of pOBK46 repaired at XbaI end inserted in EcoRI and HindIII site of pSR |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
3648 bp |
| Origin of replication/Incompatibility group: |
ColE1 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Olga Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
plasmid contains two origins of replication: ColE1 and pSC101 but replicates only from ColE1 |