Base: |
Plasmid collection |
Number in base: |
542 |
Plasmid name: |
pOBK27 |
Species: |
E.coli |
Strain: |
MC1061 recA- (EC5404) |
Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
Plasmid Sequence: |
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Construction: |
isogenic parB- control for pOBK16 carrying tryptophane promoter, 4.2-kb fragment of pOBK16 depleted of parB was obtained after digestion with PflMI and partial digestion with EcoRI, then ends were repaired and religated |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
Spc/Str |
Antibiotic concetration: |
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Plasmid size: |
ca. 4.2 kb |
Origin of replication/Incompatibility group: |
ori pSC101 |
Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
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Author: |
Olga Juszczuk |
Availability: |
non restricted |
Patent: |
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Depositor: |
O.Juszczuk |
Institution/Department: |
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Deposition date: |
2004-05-06 |
References: |
carries repAts gene for the temperature sensitive replication protein, the concentration of spc depends on quality of antibiotic (20-40 ug/ml in LB)), orientation of Ptrp towards repAts |