| Base: |
Plasmid collection |
| Number in base: |
544 |
| Plasmid name: |
pOBK28 |
| Species: |
E.coli |
| Strain: |
MC1061 recA- (EC5408) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
NsiI-MfeI fragment of pOBK12 containing the 3’ moiety of parA (84 bp) replaced by the 119 bp NsiI-MfeI fragment of PCR product obtained by amplification of the 3’ moiety of parA (692 bp) of pOBK12 with primers OMLO310 and OMLO311 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 6.4 kb |
| Origin of replication/Incompatibility group: |
ori pBR322 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Olga Juszczuk |
| Availability: |
non available |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|