| Base: |
Plasmid collection |
| Number in base: |
548 |
| Plasmid name: |
pOBK30 |
| Species: |
E.coli |
| Strain: |
C600 (EC5432 |
| Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 |
| Plasmid Sequence: |
|
| Construction: |
isogenic parA- control for pOBK29 containing egfp under the mac promoter, 145 bp BamHI-NcoI PCR fragment of parA was obtained by amplification with primers OMLO188 and OMLO189 and inserted in place of BamHI-NcoI fragment of pOBK29 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 5.96 bp |
| Origin of replication/Incompatibility group: |
ori pBR322 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
contains 96 bp from 5’ terminus of parA gene |
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|