| Base: |
Plasmid collection |
| Number in base: |
549 |
| Plasmid name: |
pOBK33 |
| Species: |
E.coli |
| Strain: |
MC1061 recA- (EC5440) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
derivative of pBEF144 containing lacZ gene in place of parPOparABS,BsrGI-SmaI fragment of PCR product obtained by amplification of lacZ gene with primers OMLO312 and OMLO313 was inserted in place of BsrGI-StyI fragment of pBEF144 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 7.97 kb |
| Origin of replication/Incompatibility group: |
ori pBR322 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|