Base: |
Plasmid collection |
Number in base: |
553 |
Plasmid name: |
λ-P1:5R kan Δ(parPO parABS) |
Species: |
E.coli |
Strain: |
DH5 Δ lac (EC5444) |
Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lac supE44 |
Plasmid Sequence: |
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Construction: |
The l-P1:5R kan Δpar was obtained by homologous recombination between the recipient l-P1:5R kan parPO parABS plasmid and HindIII-HindIII fragment of pOBK33. |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
kan |
Antibiotic concetration: |
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Plasmid size: |
ca. 45 kb |
Origin of replication/Incompatibility group: |
as plasmid – replication from P1 origin, as phage –replication from λ ori at non-permissive temperature |
Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
It carries the λ cI857; this repressor cI is temperature sensitive |
Author: |
Olga Juszczuk |
Availability: |
restricted |
Patent: |
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Depositor: |
O.Juszczuk |
Institution/Department: |
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Deposition date: |
2004-05-06 |
References: |
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