Base: |
Plasmid collection |
Number in base: |
556 |
Plasmid name: |
pOBK54 |
Species: |
E.coli |
Strain: |
C600 (EC5557) |
Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 |
Plasmid Sequence: |
|
Construction: |
derivative of pOAR3 containing mutant parAA366G (ParA K122E mutant protein) instead of wild-type parA, 1034 bp XhoI-BglII fragment of pOAR3 was replaced by XhoI-BglII fragment of pOBK53 containing parAA366G |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
amp |
Antibiotic concetration: |
|
Plasmid size: |
10167 bp |
Origin of replication/Incompatibility group: |
ori V of RSF1010 |
Host range: |
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Other genetic elements: |
|
Strain properties/Comments: |
This mutation is in the Walker A motif required for the ATPase activity |
Author: |
Olga Juszczuk |
Availability: |
non available |
Patent: |
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Depositor: |
O.Juszczuk |
Institution/Department: |
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Deposition date: |
2004-05-06 |
References: |
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