| Base: |
Plasmid collection |
| Number in base: |
557 |
| Plasmid name: |
pOBK55 |
| Species: |
E.coli |
| Strain: |
C600 (EC5559) |
| Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 |
| Plasmid Sequence: |
|
| Construction: |
derivative of pOBK31 containing mutant parAA366G-egfp (ParA K122E-Egfp mutant protein) instead of wild-type parA-egfp, 1034 bp XhoI-BglII fragment of pOBK53 containing parAA366G was inserted in place of 1034 bp XhoI-BglII fragment of pOBK31 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
ca.11.14 kb |
| Origin of replication/Incompatibility group: |
ori V of RSF1010 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
This mutation is in the Walker A motif required for the ATPase activity |
| Author: |
Olga Juszczuk |
| Availability: |
non available |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|