| Base: |
Plasmid collection |
| Number in base: |
559 |
| Plasmid name: |
λ-P1:5R kan Δ(parPO parAB) parS |
| Species: |
E.coli |
| Strain: |
C600 (EC17086) |
| Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 attB:: amp trpPO parB-gfp |
| Plasmid Sequence: |
|
| Construction: |
The λ-P1:5R kan Δ(parPO parAB) parS was obtained by homologous recombination between the recipient λ-P1:5R kan parPO parABS plasmid and linear HindIII-NruI fragment of pOBK32 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 45 kb |
| Origin of replication/Incompatibility group: |
as plasmid–replication from P1 origin, as phage–replication from λ ori at non permissive temperature |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
It carries the λ cI857; this repressor cI is temperature sensitive |
| Author: |
Olga Juszczuk |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|