Base: |
Plasmid collection |
Number in base: |
561 |
Plasmid name: |
λ-P1:5R kan Δ(parPO parAB) parS |
Species: |
E.coli |
Strain: |
C600 (EC17090) |
Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 attB:: amp trpPO parB33-gfp |
Plasmid Sequence: |
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Construction: |
The λ-P1:5R kan Δ(parPO parAB) parS was obtained by homologous recombination between the recipient λ-P1:5R kan parPO parABS plasmid and linear HindIII-NruI fragment of pOBK32. |
Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
kan |
Antibiotic concetration: |
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Plasmid size: |
ca. 45 kb |
Origin of replication/Incompatibility group: |
as plasmid–replication from P1 origin, as phage–replication from λ ori at non permissive temperature |
Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
It carries the λ cI857; this repressor cI is temperature sensitive |
Author: |
Olga Juszczuk |
Availability: |
restricted |
Patent: |
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Depositor: |
O.Juszczuk |
Institution/Department: |
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Deposition date: |
2004-05-06 |
References: |
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