| Base: |
Plasmid collection |
| Number in base: |
580 |
| Plasmid name: |
pLNO2 |
| Species: |
Escherichia coli K12 |
| Strain: |
K802 |
| Genotype: |
hsdR2 galK2 galT22 supE44
|
| Plasmid Sequence: |
not available |
| Construction: |
Ligation of the left BamHI and the right BamHI fragmentof N15cB3Cm1 linear plasmid prophage.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
cm |
| Antibiotic concetration: |
|
| Plasmid size: |
|
| Origin of replication/Incompatibility group: |
N15 plasmid prophage
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
linear mini-N15cB3 plasmid; the cB3 mutation causes the temperature sensitivity of phage repressor CB, which also controls plasmid replication. Thus the copy number of this plasmid is increased as compared to N15 prophage. |
| Author: |
Alexander Svarchevsky |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
Grigoriev and Łobocka (2001) Mol. Microbiol. 42: 355-368. |