| Base: |
Plasmid collection |
| Number in base: |
588 |
| Plasmid name: |
N15Cm1 |
| Species: |
Escherichia coli K |
| Strain: |
K802 |
| Genotype: |
hsdR2 galK2 galT22 supE44
|
| Plasmid Sequence: |
N |
| Construction: |
see reference
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Cam |
| Antibiotic concetration: |
|
| Plasmid size: |
~48 kb |
| Origin of replication/Incompatibility group: |
N15
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
N15 plasmid-prophage containing insertion of Tn9* (originally isolated by Ravin); this plasmid is maintained in cells as linear, double-stranded DNA with covalently closed ends; copy number = 5-8 per chromosome; recommended concentration of chloramphenico |
| Author: |
Alexander Svarchevsky |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2004-10-21 11:55+01:00 |
| References: |
Sankova et al. (1992) Genetika. 28: 66-76 (in Russian); ?obocka et al. (1996) J. Bacteriol. 178: 2902-2910; Ravin et al. (1999) J. Mol. Biol. 299: 53-73. |