Base: |
Plasmid collection |
Number in base: |
591 |
Plasmid name: |
pMLO161 |
Species: |
Escherichia coli K12 |
Strain: |
DJ125 |
Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
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Plasmid Sequence: |
available on request |
Construction: |
Insertion of PCR amplified PacI-BseRI fragment of pBEF116 (containing the promoter proximal moiety of parA) in place of the corresponsing PacI-BseRI fragment of pBEF116; screening for parA mutants unable to complement the partition defect of lambda-mini-P1 plasmid lacking its own parA gene. Contains parA9 mutation.
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Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
amp |
Antibiotic concetration: |
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Plasmid size: |
6.23 kb |
Origin of replication/Incompatibility group: |
pMB1 (pBR322)
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Host range: |
Escherichia coli |
Other genetic elements: |
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Strain properties/Comments: |
pBEF116 parA9; derivative of pBR322 carrying the par operon of P1 with the mutant version of parA (parA9). |
Author: |
Gayathri Arakere, Malgorzata Lobocka |
Availability: |
restricted |
Patent: |
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Depositor: |
Malgorzata Lobocka |
Institution/Department: |
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Deposition date: |
2006-07-03 09:09+01:00 |
References: |
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