| Base: |
Plasmid collection |
| Number in base: |
592 |
| Plasmid name: |
pMLO162 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Insertion of PCR amplified PacI-BseRI fragment of pBEF116 (containing the promoter distal moiety of parA) in place of the corresponsing BseRI-BseRI fragment of pBEF116; screening for parA mutants unable to complement the partition defect of lambda-mini-P1 plasmid lacking its own parA gene. Contains parA12 mutation.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
6.23 kb |
| Origin of replication/Incompatibility group: |
pMB1 (pBR322)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
pBEF116 parA12; derivative of pBR322 carrying the par operon of P1 with the mutant version of parA (parA12). |
| Author: |
Gayathri Arakere, Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
|