| Base: |
Plasmid collection |
| Number in base: |
602 |
| Plasmid name: |
pMLO45 |
| Species: |
Escherichia coli K12 |
| Strain: |
C600 |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21
|
| Plasmid Sequence: |
available on request |
| Construction: |
Insertion of 1.3 kb BamH1-SalI fragment of pMLO25 (containing the promoterles parA gene of P1) in place of BamH1-SalI fragment of pDRC150.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
~8.0 kb |
| Origin of replication/Incompatibility group: |
pMB1 (pBR322)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pBR322 carrying transcriptional fusion of P1 parA gene to the Pmac31 hybrid promoter (hybrid of promoters of maltose and lactose operons). Order of restriction sites and genmes between EcoRI site and lacZ ' in this plasmid is as follows: Ec |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
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