| Base: |
Plasmid collection |
| Number in base: |
603 |
| Plasmid name: |
pML102 |
| Species: |
Escherichia coli K12 |
| Strain: |
C600 |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21
|
| Plasmid Sequence: |
available on request |
| Construction: |
Replacement of the EcoRV-MluI fragment of pMLO87 (containing the promoter-operator region of P1 par operon, the parA gene and the 5' moiety of parB) with the SspI-MluI fragment of pBEF104 (containing the promoter-operator of trp operon of Serratia marcescens and the 5' moiety of parB).
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
~5.5 kb |
| Origin of replication/Incompatibility group: |
pMB1 (pBR322)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pBR322 carrying transcriptional fusion of P1 parB to the promoter-operator region of tryptophan operon of Serratia marcescens (trpPO), and lacking any parS sequences downstream of parB. |
| Author: |
Malgorzata Lobocka |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
Łobocka and Yarmolinsky (1996) J. Mol. Biol. 259:366--382. |