| Base: |
Plasmid collection |
| Number in base: |
604 |
| Plasmid name: |
pAJU1 |
| Species: |
Escherichia coli K12 |
| Strain: |
UT5600 |
| Genotype: |
leu proC trpE thiA rpsL Δ(fepA-ompT)
|
| Plasmid Sequence: |
available on request |
| Construction: |
Replacement of MunI-XcmI fragment of pMSK4 (encoding 5' 3/4 of P1 parB gene) with MunI-XcmI fragment of pMLO87mutB13 (encoding the corresponding fragment of mutB13.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
~5.5 kb |
| Origin of replication/Incompatibility group: |
pMB1 (pBR322)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Derivative of pBR322 carrying transcriptional fusion of P1 parB13-(his)6 to the promoter-operator region of tryptophan operon of Serratia marcescens (trpPO). Served to overexpress mutant parB13-(his)6 for protein purification. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
Adam Jujka (2001) Master' s Thesis, IBB PAS. Warsaw; Łobocka and Yarmolinsky (1996) J. Mol. Biol. 259:366--382. |