| Base: |
Plasmid collection |
| Number in base: |
612 |
| Plasmid name: |
pMLO201 |
| Species: |
Escherichia coli K12 |
| Strain: |
DH5α |
| Genotype: |
F-, Φ80dlacZ ΔM15, endA1, recA1, hsdR17 (r-,m+), supE44, thi-1, gyrA96, relA1, Δ(lacZYA-argF)U169
|
| Plasmid Sequence: |
available on request |
| Construction: |
Insertion of HindIII-AvaI fragment of pBEF116 (containing the promoter operator region of P1 par operon [parPO]), blunt-ended at the HindIII end, in place of SmaI-SalI fragment of pNM480, immediately upstream of promoterless lacZ gene
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
~5.8 kb |
| Origin of replication/Incompatibility group: |
pMB1 (pUC18)
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
Multicopy plasmid containing transcriptional fusion of P1 plasmid prophage parPO region to lacZ. Expression of lacZ from this fusion is low. |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-03 09:09+01:00 |
| References: |
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