| Base: |
Plasmid collection |
| Number in base: |
618 |
| Plasmid name: |
pAJ4 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA) |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request |
| Construction: |
Constructed by replacement of PvuI-NsiI fragment of pAJ3 containing the pBR322 origin of replication and a part of bla gene by 1529 bp PvuI-NsiI fragment obtained by PCR amplification and carrying the loxP cre cassette of P1.
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
cam |
| Antibiotic concetration: |
|
| Plasmid size: |
|
| Origin of replication/Incompatibility group: |
P7 prophage
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
This plasmid is a mini-P7 containing the P1 loxP cre cassette. It is driven by P7 origin of replication and is stably maintained in cells as a unit copy plasmid. It contains the partition cassette of P7. DJ125/pAJ4=EC15093 |
| Author: |
Aleksandra Janowicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 18:33+01:00 |
| References: |
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