| Base: |
Plasmid collection |
| Number in base: |
621 |
| Plasmid name: |
pMLO14 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 |
| Genotype: |
supE44, hsdR, thi-1, leuB6, lacY1, thr-1, tonA21, recA56
|
| Plasmid Sequence: |
N
|
| Construction: |
Replacement of HindIII-EcoRV fragment of pBR322 by HindIII-SspI fragment (3.86 kb) of pGB2ts containing ori, repAts and specR gene of pGB2ts
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
spc/str |
| Antibiotic concetration: |
|
| Plasmid size: |
8.03 kb |
| Origin of replication/Incompatibility group: |
pSC101, pMB1
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
double origin plasmid, contains temperature sensitive repA allele of pSC101; at 37oC can replicate only from pMB1 origin |
| Author: |
Malgorzata Lobocka |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
M. Lobocka |
| Institution/Department: |
|
| Deposition date: |
2005-02-01 17:04+01:00 |
| References: |
unpublished |