Base: |
Plasmid collection |
Number in base: |
622 |
Plasmid name: |
pMLO19 |
Species: |
Escherichia coli K12 |
Strain: |
DJ125 (=C600 recA); EC16825 |
Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
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Plasmid Sequence: |
available on request
|
Construction: |
Ligation of HindIII-BamHI fragment of pGB2ts with the large HindIII-BamH1 fragment of pBR322
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Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
spc; str; amp |
Antibiotic concetration: |
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Plasmid size: |
~4 kb |
Origin of replication/Incompatibility group: |
pSC101; pMB1
|
Host range: |
Escherichia coli |
Other genetic elements: |
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Strain properties/Comments: |
This plasmid is a hybrid of pBR322 and pGB2ts. At elevated temperature (42oC) it can replicate only as a high-copy number plasmid from the pMB1 origin of replication. |
Author: |
Malgorzata Lobocka |
Availability: |
non restricted |
Patent: |
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Depositor: |
Malgorzata Lobocka |
Institution/Department: |
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Deposition date: |
2006-07-02 12:09+01:00 |
References: |
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